MR lymphography with iron oxide compound AMI-227

Studies in ferrets with filariasis

T. Tanoura, Michael J Bernas, A. Darkazanli, E. Elam, Evan C Unger, Marlys H Witte, A. Green

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

OBJECTIVE. The purpose of this study was to assess the MR lymphographic potential of AMI-227 to reduce the signal intensity of hyperplastic inflammatory lymph nodes by using ferrets with filariasis as the animal model. Both interstitial and IV modes of administration were studied. MATERIALS AND METHODS. Twelve ferrets were infected with the filaria Brugia malayi and six control ferrets were injected with superparamagnetic iron oxide AMI-227, either interstitially or IV. Signal intensities of the left popliteal lymph node, left hamstring muscle, and left inguinal fat were measured before, 48 hr after, and up to 138 days after contrast injection with the use of both spin-echo and gradient-echo techniques. The signal intensity data were statistically analyzed. The lymph nodes, liver, spleen, and lungs were studied with light and electron microscopy. RESULTS. Forty- eight hours after the interstitial injection of AMI-227, the signal intensities of the ipsilateral popliteal nodes of the infected and control ferrets were significantly reduced (p < .0005) without significant changes in the signal intensities of the surrounding tissues on both spin-echo and gradient-echo MR images. No nodal signal reduction occurred with the IV route of injection in ferrets. Light microscopy revealed iron to be localized within nodal marginal zones exclusively. Lymphatic trunks were visualized after interstitial injection. Signal reduction persisted to our end point of 138 days. CONCLUSION. AMI-227 shows regional specificity with significant enhancement of nodal structures and demonstrates potential as an interstitial MR lymphographic agent.

Original languageEnglish (US)
Pages (from-to)875-881
Number of pages7
JournalAmerican Journal of Roentgenology
Volume159
Issue number4
StatePublished - 1992

Fingerprint

Iron Compounds
Filariasis
Lymphography
Ferrets
Injections
Lymph Nodes
Brugia malayi
Light
Groin
Microscopy
Electron Microscopy
Spleen
Iron
Animal Models
Fats
ferric oxide
ferumoxtran-10
Lung
Liver

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

MR lymphography with iron oxide compound AMI-227 : Studies in ferrets with filariasis. / Tanoura, T.; Bernas, Michael J; Darkazanli, A.; Elam, E.; Unger, Evan C; Witte, Marlys H; Green, A.

In: American Journal of Roentgenology, Vol. 159, No. 4, 1992, p. 875-881.

Research output: Contribution to journalArticle

@article{ca5b8b8fa7744958971b2f85754e16e1,
title = "MR lymphography with iron oxide compound AMI-227: Studies in ferrets with filariasis",
abstract = "OBJECTIVE. The purpose of this study was to assess the MR lymphographic potential of AMI-227 to reduce the signal intensity of hyperplastic inflammatory lymph nodes by using ferrets with filariasis as the animal model. Both interstitial and IV modes of administration were studied. MATERIALS AND METHODS. Twelve ferrets were infected with the filaria Brugia malayi and six control ferrets were injected with superparamagnetic iron oxide AMI-227, either interstitially or IV. Signal intensities of the left popliteal lymph node, left hamstring muscle, and left inguinal fat were measured before, 48 hr after, and up to 138 days after contrast injection with the use of both spin-echo and gradient-echo techniques. The signal intensity data were statistically analyzed. The lymph nodes, liver, spleen, and lungs were studied with light and electron microscopy. RESULTS. Forty- eight hours after the interstitial injection of AMI-227, the signal intensities of the ipsilateral popliteal nodes of the infected and control ferrets were significantly reduced (p < .0005) without significant changes in the signal intensities of the surrounding tissues on both spin-echo and gradient-echo MR images. No nodal signal reduction occurred with the IV route of injection in ferrets. Light microscopy revealed iron to be localized within nodal marginal zones exclusively. Lymphatic trunks were visualized after interstitial injection. Signal reduction persisted to our end point of 138 days. CONCLUSION. AMI-227 shows regional specificity with significant enhancement of nodal structures and demonstrates potential as an interstitial MR lymphographic agent.",
author = "T. Tanoura and Bernas, {Michael J} and A. Darkazanli and E. Elam and Unger, {Evan C} and Witte, {Marlys H} and A. Green",
year = "1992",
language = "English (US)",
volume = "159",
pages = "875--881",
journal = "American Journal of Roentgenology",
issn = "0361-803X",
publisher = "American Roentgen Ray Society",
number = "4",

}

TY - JOUR

T1 - MR lymphography with iron oxide compound AMI-227

T2 - Studies in ferrets with filariasis

AU - Tanoura, T.

AU - Bernas, Michael J

AU - Darkazanli, A.

AU - Elam, E.

AU - Unger, Evan C

AU - Witte, Marlys H

AU - Green, A.

PY - 1992

Y1 - 1992

N2 - OBJECTIVE. The purpose of this study was to assess the MR lymphographic potential of AMI-227 to reduce the signal intensity of hyperplastic inflammatory lymph nodes by using ferrets with filariasis as the animal model. Both interstitial and IV modes of administration were studied. MATERIALS AND METHODS. Twelve ferrets were infected with the filaria Brugia malayi and six control ferrets were injected with superparamagnetic iron oxide AMI-227, either interstitially or IV. Signal intensities of the left popliteal lymph node, left hamstring muscle, and left inguinal fat were measured before, 48 hr after, and up to 138 days after contrast injection with the use of both spin-echo and gradient-echo techniques. The signal intensity data were statistically analyzed. The lymph nodes, liver, spleen, and lungs were studied with light and electron microscopy. RESULTS. Forty- eight hours after the interstitial injection of AMI-227, the signal intensities of the ipsilateral popliteal nodes of the infected and control ferrets were significantly reduced (p < .0005) without significant changes in the signal intensities of the surrounding tissues on both spin-echo and gradient-echo MR images. No nodal signal reduction occurred with the IV route of injection in ferrets. Light microscopy revealed iron to be localized within nodal marginal zones exclusively. Lymphatic trunks were visualized after interstitial injection. Signal reduction persisted to our end point of 138 days. CONCLUSION. AMI-227 shows regional specificity with significant enhancement of nodal structures and demonstrates potential as an interstitial MR lymphographic agent.

AB - OBJECTIVE. The purpose of this study was to assess the MR lymphographic potential of AMI-227 to reduce the signal intensity of hyperplastic inflammatory lymph nodes by using ferrets with filariasis as the animal model. Both interstitial and IV modes of administration were studied. MATERIALS AND METHODS. Twelve ferrets were infected with the filaria Brugia malayi and six control ferrets were injected with superparamagnetic iron oxide AMI-227, either interstitially or IV. Signal intensities of the left popliteal lymph node, left hamstring muscle, and left inguinal fat were measured before, 48 hr after, and up to 138 days after contrast injection with the use of both spin-echo and gradient-echo techniques. The signal intensity data were statistically analyzed. The lymph nodes, liver, spleen, and lungs were studied with light and electron microscopy. RESULTS. Forty- eight hours after the interstitial injection of AMI-227, the signal intensities of the ipsilateral popliteal nodes of the infected and control ferrets were significantly reduced (p < .0005) without significant changes in the signal intensities of the surrounding tissues on both spin-echo and gradient-echo MR images. No nodal signal reduction occurred with the IV route of injection in ferrets. Light microscopy revealed iron to be localized within nodal marginal zones exclusively. Lymphatic trunks were visualized after interstitial injection. Signal reduction persisted to our end point of 138 days. CONCLUSION. AMI-227 shows regional specificity with significant enhancement of nodal structures and demonstrates potential as an interstitial MR lymphographic agent.

UR - http://www.scopus.com/inward/record.url?scp=0026656377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026656377&partnerID=8YFLogxK

M3 - Article

VL - 159

SP - 875

EP - 881

JO - American Journal of Roentgenology

JF - American Journal of Roentgenology

SN - 0361-803X

IS - 4

ER -