MUC1 is a counter-receptor for myelin-associated glycoprotein (Siglec-4a) and their interaction contributes to adhesion in pancreatic cancer perineural invasion

Benjamin J. Swanson, Kimberly McDermott, Pankaj K. Singh, John P. Eggers, Paul R. Crocker, Michael A. Hollingsworth

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Perineural invasion in pancreatic adenocarcinoma, a common pathologic phenomenon whereby cancer cells invade and intimately contact the endoneurium of pancreatic nerves, is thought to contribute to both pain and local disease recurrence. MUC1, a type I transmembrane mucin that can affect the adhesive properties of cells, contains a large extracellular tandem repeat domain, which is heavily glycosylated in normal epithelia, but is overexpressed and differentially glycosylated in pancreatic cancer. This altered glycosylation includes the shortened core I O-glycans for monosialyl and disialyl Tantigens. Myelin-associated glycoprotein (MAG), a membrane-bound protein expressed on oligodendrocytes and Schwann cells, binds myelin to neurons. MAG's preferred ligands are derivatives of the monosialyl and disialyl Tantigen. We investigated whether MUC1 is a counter-receptor for MAG and if their interaction contributed to pancreatic perineural invasion. Results showed that MAG binds pancreatic cells expressing MUC1, that this binding is sialidase-sensitive, and that MAG physically associates with MUC1. Heterotypic adhesion assays between pancreatic cancer cells and Schwann cells revealed that increased expression of MUC1 or MAG enhanced adhesion. Conversely, specific inhibition of MAG or sialyl-TM UC1 partially blocked adhesion. Immunohistochemical analysis of pancreatic perineural invasion showed the expression of both MUC1 and MAG. These results support the hypothesis that the adhesive interactions between MUC1 and MAG are of biological significance in pancreatic cancer perineural invasion.

Original languageEnglish (US)
Pages (from-to)10222-10229
Number of pages8
JournalCancer Research
Volume67
Issue number21
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

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Sialic Acid Binding Immunoglobulin-like Lectins
Myelin-Associated Glycoprotein
Pancreatic Neoplasms
Schwann Cells
Adhesives
Tandem Repeat Sequences
Oligodendroglia
Neuraminidase
Mucins
Myelin Sheath
Glycosylation
Peripheral Nerves
Polysaccharides
Membrane Proteins
Adenocarcinoma
Epithelium
Ligands
Neurons
Recurrence
Pain

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

MUC1 is a counter-receptor for myelin-associated glycoprotein (Siglec-4a) and their interaction contributes to adhesion in pancreatic cancer perineural invasion. / Swanson, Benjamin J.; McDermott, Kimberly; Singh, Pankaj K.; Eggers, John P.; Crocker, Paul R.; Hollingsworth, Michael A.

In: Cancer Research, Vol. 67, No. 21, 01.11.2007, p. 10222-10229.

Research output: Contribution to journalArticle

Swanson, Benjamin J. ; McDermott, Kimberly ; Singh, Pankaj K. ; Eggers, John P. ; Crocker, Paul R. ; Hollingsworth, Michael A. / MUC1 is a counter-receptor for myelin-associated glycoprotein (Siglec-4a) and their interaction contributes to adhesion in pancreatic cancer perineural invasion. In: Cancer Research. 2007 ; Vol. 67, No. 21. pp. 10222-10229.
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