Mucolipidosis type IV protein TRPML1-dependent lysosome formation

Austin Miller, Jessica Schafer, Cameron Upchurch, Ellen Spooner, Julie Huynh, Sebastian Hernandez, Brooke Mclaughlin, Liam Oden, Hanna Fares

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Lysosomes are dynamic organelles that undergo cycles of fusion and fission with themselves and with other organelles. Following fusion with late endosomes to form hybrid organelles, lysosomes are reformed as discrete organelles. This lysosome reformation or formation is a poorly understood process that has not been systematically analyzed and that lacks known regulators. In this study, we quantitatively define the multiple steps of lysosome formation and identify the first regulator of this process. Lysosomes are dynamic organelles that undergo cycles of fusion and fission with themselves and with other organelles. New lysosomes are formed or reformed through a process of budding from late endosomes/hybrid organelles, membrane extension moving the nascent lysosomes away from the late endosomes late endosomes/hybrid organelles, and scission of the connecting membrane releasing the lysosomes. We show that the Mucolipidosis type IV non-selective cation channel protein TRPML1 is required for the scission step that releases distinct lysosomes.

Original languageEnglish (US)
Pages (from-to)284-297
Number of pages14
JournalTraffic
Volume16
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • Lysosome
  • Mucolipidosis type IV
  • TRPML1

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Mucolipidosis type IV protein TRPML1-dependent lysosome formation'. Together they form a unique fingerprint.

  • Cite this

    Miller, A., Schafer, J., Upchurch, C., Spooner, E., Huynh, J., Hernandez, S., Mclaughlin, B., Oden, L., & Fares, H. (2015). Mucolipidosis type IV protein TRPML1-dependent lysosome formation. Traffic, 16(3), 284-297. https://doi.org/10.1111/tra.12249