Multi-color single filter photobleaching analysis for measuring gene expression

Pak Kin Wong, L. J. Chun, C. M. Ho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Fluorescence microscopy is widely used in biomedical applications to quantitatively measure specific molecular signatures. It is often valuable to study several biosignatures simultaneously, such as in early stages of cancer diagnostics. Currently, multiple analyte can be observed based on the excitation and emission spectra of fluorescent dyes. However, the number of concurrent dyes is limited by the optical spectral bandwidth. We have developed a novel identification technique based on the photostability of different fluorophores. Fluorophore identification was demonstrated in mixtures of rhodamine 6B and rhodamine 6G. In addition, the concentration of each dye was successfully determined. Since the analysis is independent of the individual dye spectra, this technology can be easily integrated with existing spectra-based systems for expanding the ability of multi-analyte detection.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages629-630
Number of pages2
Volume1
Publication statusPublished - 2002
Externally publishedYes
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

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Keywords

  • Gene expressions
  • Immunofluorescence
  • Multiple analytes
  • Photobleaching

ASJC Scopus subject areas

  • Bioengineering

Cite this

Wong, P. K., Chun, L. J., & Ho, C. M. (2002). Multi-color single filter photobleaching analysis for measuring gene expression. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 629-630)