Multi-parametric analysis of cellular and subcellular function using spectral imaging

Ron Lynch, Kevin D. Nullmeyer, Bridget K. Ford, Linda S. Tompkins, Vicki L. Sutherland, Michael R. Descour

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The development of fluorescent probes that sense cellular constituents has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques that simultaneously monitor signal from multiple probes with high temporal resolution are required. This article discusses the use of spectral imaging approaches for simultaneous analysis of pH and Ca2+ in singlet cells.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
Pages79-87
Number of pages9
Volume3924
Publication statusPublished - 2000
EventMolecular Imaging: Reporters, Dyes, Markers, and Instrumentation - San Jose, CA, USA
Duration: Jan 23 2000Jan 24 2000

Other

OtherMolecular Imaging: Reporters, Dyes, Markers, and Instrumentation
CitySan Jose, CA, USA
Period1/23/001/24/00

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Lynch, R., Nullmeyer, K. D., Ford, B. K., Tompkins, L. S., Sutherland, V. L., & Descour, M. R. (2000). Multi-parametric analysis of cellular and subcellular function using spectral imaging. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3924, pp. 79-87). SPIE.