Multi-parametric analysis of cellular and subcellular function using spectral imaging

Ronald M. Lynch, Kevin D. Nullmeyer, Bridget K. Ford, Linda S. Tompkins, Vicki L. Sutherland, Michael R. Descour

Research output: Contribution to journalConference article

2 Scopus citations

Abstract

The development of fluorescent probes that sense cellular constituents has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques that simultaneously monitor signal from multiple probes with high temporal resolution are required. This article discusses the use of spectral imaging approaches for simultaneous analysis of pH and Ca2+ in singlet cells.

Original languageEnglish (US)
Pages (from-to)79-87
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3924
StatePublished - Jan 1 2000
EventMolecular Imaging: Reporters, Dyes, Markers, and Instrumentation - San Jose, CA, USA
Duration: Jan 23 2000Jan 24 2000

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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