Multi-wavelength quantitative polarization and phase microscope

Xiaobo Tian, Xingzhou Tu, Kimiko Della Croce, Guang Yao, Haijiang Cai, Neal Brock, Stanley K H Pau, Rongguang Liang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We introduce a snapshot multi-wavelength quantitative polarization and phase microscope (MQPPM) for measuring spectral dependent quantitative polarization and phase information. The system uniquely integrates a polarized light microscope and a snap-shot quantitative phase microscope in a single system, utilizing a novel full-Stokes camera operating in the red, green, and blue (RGB) spectrum. The linear retardance and fast axis orientation of a birefringent sample can be measured simultaneously in the visible spectra. Both theoretical analysis and experiments have been performed to demonstrate the capability of the proposed microscope. Data from liquid crystal and different biological samples are presented. We believe that MQPPM will be a useful tool in measuring quantitative polarization and phase information of live cells.

Original languageEnglish (US)
Pages (from-to)1638-1648
Number of pages11
JournalBiomedical Optics Express
Volume10
Issue number4
DOIs
StatePublished - Apr 1 2019

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Liquid Crystals
microscopes
Light
polarization
wavelengths
visible spectrum
polarized light
shot
liquid crystals
cameras
cells

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Multi-wavelength quantitative polarization and phase microscope. / Tian, Xiaobo; Tu, Xingzhou; Croce, Kimiko Della; Yao, Guang; Cai, Haijiang; Brock, Neal; Pau, Stanley K H; Liang, Rongguang.

In: Biomedical Optics Express, Vol. 10, No. 4, 01.04.2019, p. 1638-1648.

Research output: Contribution to journalArticle

Tian, Xiaobo ; Tu, Xingzhou ; Croce, Kimiko Della ; Yao, Guang ; Cai, Haijiang ; Brock, Neal ; Pau, Stanley K H ; Liang, Rongguang. / Multi-wavelength quantitative polarization and phase microscope. In: Biomedical Optics Express. 2019 ; Vol. 10, No. 4. pp. 1638-1648.
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