Multifocal electroretinography in isolated arterially perfused bovine eye

Mohammad - Shahidullah, Henry H L Chan, Maurice K. Yap, Quan Liu, Chi Ho To

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim: To develop an isolated arterially perfused bovine eye as an in vitro model for studying retinal electrophysiology using multifocal electroretinography (mfERG). Methods: Fresh bovine eyes were cannulated through the ophthalmic artery and perfused with oxygenated Krebs' solution at 37°C within 15 min postmortem. Multifocal ERG was recorded using the VERIS 1.0 (Visual Evoked Response Imaging System). Results: The mfERG waveform of in vitro bovine eyes had a typical trough (negative wave, N1) and peak (positive wave, P1) similar to those of human mfERG. The implicit times (peak latencies) for N1 and P1 were 20 and 42 ms respectively, and the average response amplitude (N1P1) was 55 nV. The implicit times of N1 and P1 were maintained for more than 4 h. The mfERG responses were affected proportionately by the stimulus intensity. Without a neutral density filter, the mean response amplitude was about 55 nV, which was diminished to 26,18 and 10 nV under neutral density filters of 0.4, 1.0 and 1.4, respectively. Partial occlusion of the pupil completely abolished mfERG responses from the corresponding part of the retina. Conclusions: Isolated arterially perfused bovine eye may be used as an experimental model to study retinal electrophysiology using mfERG. The in vitro eye may offer a convenient and easily obtainable alternative to live animals, particularly for drug screening and invasive studies.

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalOphthalmic and Physiological Optics
Volume25
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

Electroretinography
Electrophysiology
Ophthalmic Artery
Preclinical Drug Evaluations
Visual Evoked Potentials
Pupil
Evoked Potentials
Retina
Theoretical Models
In Vitro Techniques

Keywords

  • Bovine eye
  • Electroretinogram
  • In vitro model
  • Multifocal electroretinography
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Health Professions(all)

Cite this

Multifocal electroretinography in isolated arterially perfused bovine eye. / Shahidullah, Mohammad -; Chan, Henry H L; Yap, Maurice K.; Liu, Quan; To, Chi Ho.

In: Ophthalmic and Physiological Optics, Vol. 25, No. 1, 01.2005, p. 27-34.

Research output: Contribution to journalArticle

Shahidullah, Mohammad - ; Chan, Henry H L ; Yap, Maurice K. ; Liu, Quan ; To, Chi Ho. / Multifocal electroretinography in isolated arterially perfused bovine eye. In: Ophthalmic and Physiological Optics. 2005 ; Vol. 25, No. 1. pp. 27-34.
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