Multimeric C9 within C5b-9 deposits in unique locations in the cell wall of Salmonella typhimurium

Keith A Joiner, A. B. Tartanian, C. H. Hammer, J. E. Schweinle

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have shown previously that multimeric C9 within C5b-9 (C9:C5b-8 > 3:1) is needed for killing of a rough strain of Escherichia coli. We now extend these studies using serum sensitive, rough (R) and serum resistant, wild type (WT) strains of Salmonella typhimurium as well as a mutant S. typhimurium strain (TS) with a temperature sensitive mutation in synthesis of keto-deoxy-octulosonate, a constituent within the deep core structure of Salmonella LPS. Both R and TS required multimeric C9 within C5b-9 to be killed. Addition at 37°C of increasing inputs of C9 to TS or R bearing C5b-9 led to a dose-related increase in C9 binding and killing. In contrast, addition of high inputs of C9 to the same strains at 4°C, a procedure that limits the C9:C5b-8 ratio to 1:1, resulted in low C9 binding and minimal killing. Bactericidal C5b-9 formed at 37°C on R and TS with high inputs of C9 co-sedimented with the bacterial outer membrane on sucrose density gradient analysis. Non-bactericidal C5b-9 on R, WT, and TS co-sedimented near the inner membrane, despite the presumed lack of association between these constituents. Whereas 125I C9 within the non-bactericidal pools immunoprecipitated with anti-C5, 125I C9 within bactericidal pools did not immunoprecipitate with anti-C5, anti-C7, or anti-C9. These findings suggest that bactericidal C5b-9 may be deposited in a unique location or configuration within the bacterial cell wall.

Original languageEnglish (US)
Pages (from-to)4450-4457
Number of pages8
JournalJournal of Immunology
Volume142
Issue number12
StatePublished - 1989
Externally publishedYes

Fingerprint

Complement Membrane Attack Complex
Salmonella typhimurium
Cell Wall
Membranes
Serum
Salmonella
Sucrose
Escherichia coli
Mutation
Temperature

ASJC Scopus subject areas

  • Immunology

Cite this

Multimeric C9 within C5b-9 deposits in unique locations in the cell wall of Salmonella typhimurium. / Joiner, Keith A; Tartanian, A. B.; Hammer, C. H.; Schweinle, J. E.

In: Journal of Immunology, Vol. 142, No. 12, 1989, p. 4450-4457.

Research output: Contribution to journalArticle

Joiner, KA, Tartanian, AB, Hammer, CH & Schweinle, JE 1989, 'Multimeric C9 within C5b-9 deposits in unique locations in the cell wall of Salmonella typhimurium', Journal of Immunology, vol. 142, no. 12, pp. 4450-4457.
Joiner, Keith A ; Tartanian, A. B. ; Hammer, C. H. ; Schweinle, J. E. / Multimeric C9 within C5b-9 deposits in unique locations in the cell wall of Salmonella typhimurium. In: Journal of Immunology. 1989 ; Vol. 142, No. 12. pp. 4450-4457.
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