Multimodality imaging in an orthotopic mammary window chamber model

Rachel Schafer, Hui Min Leung, Arthur F Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Window chamber models have been utilized for many years to investigate cancer development and the tumor microenvironment. Orthotopic mammary window chamber model have been developed for detailed study of breast cancer. Orthotopic window chamber models, due to the native environment, support more realistic growth and tumor behavior than ectopic models. The work by other groups thus far utilizing mammary window chamber models has focused solely on optical imaging techniques, limited to probing the first millimeter or less of tissue. These techniques do not take full advantage of the unrestricted, three-dimensional tumor growth the model supports. We have developed a custom plastic structure compatible with multimodality imaging. We present in this work the implementation of our custom window chamber in a mouse model and the successful imaging of the window chamber cancer model with MRI, nuclear imaging, and optical techniques. MRI provides a full three-dimensional view of the tumor growth and allows for additional, potentially clinically translatable, approaches to be utilized in investigating the cancer microenvironment. Nuclear imaging is accomplished using the Beta Imager, which is a novel approach to nuclear imaging of window chambers. The Beta Imager detects photons after the interaction of a single positron with a scintillator, instead of the coincidence detection of annihilation gamma ray pairs. We utilized the radioisotope glucose analog, 2-deoxy-2- (18F)fluoro-D-glucose or FDG, with the Beta Imager to obtain information on the glycolytic metabolism of the tumor and surrounding region.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8587
DOIs
StatePublished - 2013
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI - San Francisco, CA, United States
Duration: Feb 2 2013Feb 5 2013

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
CountryUnited States
CitySan Francisco, CA
Period2/2/132/5/13

Fingerprint

Breast
chambers
Imaging techniques
Tumors
tumors
Neoplasms
Tumor Microenvironment
cancer
Optical Imaging
Image sensors
Growth
glucose
Magnetic resonance imaging
Glucose
Gamma Rays
Fluorodeoxyglucose F18
Photons
Radioisotopes
Plastics
Positrons

Keywords

  • cancer microenvironment
  • Multimodality imaging
  • window chamber

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Schafer, R., Leung, H. M., & Gmitro, A. F. (2013). Multimodality imaging in an orthotopic mammary window chamber model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8587). [858702] https://doi.org/10.1117/12.2004973

Multimodality imaging in an orthotopic mammary window chamber model. / Schafer, Rachel; Leung, Hui Min; Gmitro, Arthur F.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8587 2013. 858702.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Schafer, R, Leung, HM & Gmitro, AF 2013, Multimodality imaging in an orthotopic mammary window chamber model. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8587, 858702, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, San Francisco, CA, United States, 2/2/13. https://doi.org/10.1117/12.2004973
Schafer R, Leung HM, Gmitro AF. Multimodality imaging in an orthotopic mammary window chamber model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8587. 2013. 858702 https://doi.org/10.1117/12.2004973
Schafer, Rachel ; Leung, Hui Min ; Gmitro, Arthur F. / Multimodality imaging in an orthotopic mammary window chamber model. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8587 2013.
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