Multimodality pH imaging in a mouse dorsal skin fold window chamber model

Hui Min Leung, Rachel Schafer, Mark M. Pagel, Ian F. Robey, Arthur F. Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Upregulate levels of expression and activity of membrane H+ ion pumps in cancer cells drives the extracellular pH (pHe,) to values lower than normal. Furthermore, disregulated pH is indicative of the changes in glycolytic metabolism in tumor cells and has been shown to facilitate extracellular tissue remodeling during metastasis Therefore, measurement of pHe could be a useful cancer biomarker for diagnostic and therapy monitoring evaluation. Multimodality in-vivo imaging of pHe in tumorous tissue in a mouse dorsal skin fold window chamber (DSFWC) model is described. A custom-made plastic window chamber structure was developed that is compatible with both imaging optical and MR imaging modalities and provides a model system for continuous study of the same tissue microenvironment on multiple imaging platforms over a 3-week period. For optical imaging of pH e, SNARF-1 carboxylic acid is injected intravenously into a SCID mouse with an implanted tumor. A ratiometric measurement of the fluorescence signal captured on a confocal microscope reveals the pHe of the tissue visible within the window chamber. This imaging method was used in a preliminary study to evaluate sodium bicarbonate as a potential drug treatment to reverse tissue acidosis. For MR imaging of pHe the chemical exchange saturation transfer (CEST) was used as an alternative way of measuring pHe in a DSFWC model. ULTRAVIST®, a FDA approved x-ray/CT contrast agent has been shown to have a CEST effect that is pH dependent. A ratiometric analysis of water saturation at 5.6 and 4.2 ppm chemical shift provides a means to estimate the local pHe.

Original languageEnglish (US)
Title of host publicationMultimodal Biomedical Imaging VIII
DOIs
StatePublished - May 22 2013
EventMultimodal Biomedical Imaging VIII - San Francisco, CA, United States
Duration: Feb 2 2013Feb 2 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8574
ISSN (Print)1605-7422

Other

OtherMultimodal Biomedical Imaging VIII
CountryUnited States
CitySan Francisco, CA
Period2/2/132/2/13

Keywords

  • CEST
  • MRI
  • acidosis
  • cancer
  • confocal
  • fluorescence
  • in vivo imaging
  • microenvironment
  • pH
  • window chamber

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Leung, H. M., Schafer, R., Pagel, M. M., Robey, I. F., & Gmitro, A. F. (2013). Multimodality pH imaging in a mouse dorsal skin fold window chamber model. In Multimodal Biomedical Imaging VIII [85740L] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8574). https://doi.org/10.1117/12.2005472