Multimodality pH imaging in a mouse dorsal skin fold window chamber model

Hui Min Leung, Rachel Schafer, Mark "Marty" Pagel, Ian F. Robey, Arthur F Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Upregulate levels of expression and activity of membrane H+ ion pumps in cancer cells drives the extracellular pH (pHe,) to values lower than normal. Furthermore, disregulated pH is indicative of the changes in glycolytic metabolism in tumor cells and has been shown to facilitate extracellular tissue remodeling during metastasis Therefore, measurement of pHe could be a useful cancer biomarker for diagnostic and therapy monitoring evaluation. Multimodality in-vivo imaging of pHe in tumorous tissue in a mouse dorsal skin fold window chamber (DSFWC) model is described. A custom-made plastic window chamber structure was developed that is compatible with both imaging optical and MR imaging modalities and provides a model system for continuous study of the same tissue microenvironment on multiple imaging platforms over a 3-week period. For optical imaging of pH e, SNARF-1 carboxylic acid is injected intravenously into a SCID mouse with an implanted tumor. A ratiometric measurement of the fluorescence signal captured on a confocal microscope reveals the pHe of the tissue visible within the window chamber. This imaging method was used in a preliminary study to evaluate sodium bicarbonate as a potential drug treatment to reverse tissue acidosis. For MR imaging of pHe the chemical exchange saturation transfer (CEST) was used as an alternative way of measuring pHe in a DSFWC model. ULTRAVIST®, a FDA approved x-ray/CT contrast agent has been shown to have a CEST effect that is pH dependent. A ratiometric analysis of water saturation at 5.6 and 4.2 ppm chemical shift provides a means to estimate the local pHe.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8574
DOIs
StatePublished - 2013
EventMultimodal Biomedical Imaging VIII - San Francisco, CA, United States
Duration: Feb 2 2013Feb 2 2013

Other

OtherMultimodal Biomedical Imaging VIII
CountryUnited States
CitySan Francisco, CA
Period2/2/132/2/13

Fingerprint

mice
Skin
chambers
Imaging techniques
Tissue
Optical Imaging
saturation
acidosis
tumors
cancer
Ion Pumps
ion pumps
Proton Pumps
Neoplasms
Tumors
Sodium Bicarbonate
SCID Mice
biomarkers
metastasis
metabolism

Keywords

  • acidosis
  • cancer
  • CEST
  • confocal
  • fluorescence
  • in vivo imaging
  • microenvironment
  • MRI
  • pH
  • window chamber

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Leung, H. M., Schafer, R., Pagel, M. M., Robey, I. F., & Gmitro, A. F. (2013). Multimodality pH imaging in a mouse dorsal skin fold window chamber model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8574). [85740L] https://doi.org/10.1117/12.2005472

Multimodality pH imaging in a mouse dorsal skin fold window chamber model. / Leung, Hui Min; Schafer, Rachel; Pagel, Mark "Marty"; Robey, Ian F.; Gmitro, Arthur F.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8574 2013. 85740L.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Leung, HM, Schafer, R, Pagel, MM, Robey, IF & Gmitro, AF 2013, Multimodality pH imaging in a mouse dorsal skin fold window chamber model. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8574, 85740L, Multimodal Biomedical Imaging VIII, San Francisco, CA, United States, 2/2/13. https://doi.org/10.1117/12.2005472
Leung HM, Schafer R, Pagel MM, Robey IF, Gmitro AF. Multimodality pH imaging in a mouse dorsal skin fold window chamber model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8574. 2013. 85740L https://doi.org/10.1117/12.2005472
Leung, Hui Min ; Schafer, Rachel ; Pagel, Mark "Marty" ; Robey, Ian F. ; Gmitro, Arthur F. / Multimodality pH imaging in a mouse dorsal skin fold window chamber model. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8574 2013.
@inproceedings{c24d84c4b507444c85e3250b42c635f6,
title = "Multimodality pH imaging in a mouse dorsal skin fold window chamber model",
abstract = "Upregulate levels of expression and activity of membrane H+ ion pumps in cancer cells drives the extracellular pH (pHe,) to values lower than normal. Furthermore, disregulated pH is indicative of the changes in glycolytic metabolism in tumor cells and has been shown to facilitate extracellular tissue remodeling during metastasis Therefore, measurement of pHe could be a useful cancer biomarker for diagnostic and therapy monitoring evaluation. Multimodality in-vivo imaging of pHe in tumorous tissue in a mouse dorsal skin fold window chamber (DSFWC) model is described. A custom-made plastic window chamber structure was developed that is compatible with both imaging optical and MR imaging modalities and provides a model system for continuous study of the same tissue microenvironment on multiple imaging platforms over a 3-week period. For optical imaging of pH e, SNARF-1 carboxylic acid is injected intravenously into a SCID mouse with an implanted tumor. A ratiometric measurement of the fluorescence signal captured on a confocal microscope reveals the pHe of the tissue visible within the window chamber. This imaging method was used in a preliminary study to evaluate sodium bicarbonate as a potential drug treatment to reverse tissue acidosis. For MR imaging of pHe the chemical exchange saturation transfer (CEST) was used as an alternative way of measuring pHe in a DSFWC model. ULTRAVIST{\circledR}, a FDA approved x-ray/CT contrast agent has been shown to have a CEST effect that is pH dependent. A ratiometric analysis of water saturation at 5.6 and 4.2 ppm chemical shift provides a means to estimate the local pHe.",
keywords = "acidosis, cancer, CEST, confocal, fluorescence, in vivo imaging, microenvironment, MRI, pH, window chamber",
author = "Leung, {Hui Min} and Rachel Schafer and Pagel, {Mark {"}Marty{"}} and Robey, {Ian F.} and Gmitro, {Arthur F}",
year = "2013",
doi = "10.1117/12.2005472",
language = "English (US)",
isbn = "9780819493439",
volume = "8574",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Multimodality pH imaging in a mouse dorsal skin fold window chamber model

AU - Leung, Hui Min

AU - Schafer, Rachel

AU - Pagel, Mark "Marty"

AU - Robey, Ian F.

AU - Gmitro, Arthur F

PY - 2013

Y1 - 2013

N2 - Upregulate levels of expression and activity of membrane H+ ion pumps in cancer cells drives the extracellular pH (pHe,) to values lower than normal. Furthermore, disregulated pH is indicative of the changes in glycolytic metabolism in tumor cells and has been shown to facilitate extracellular tissue remodeling during metastasis Therefore, measurement of pHe could be a useful cancer biomarker for diagnostic and therapy monitoring evaluation. Multimodality in-vivo imaging of pHe in tumorous tissue in a mouse dorsal skin fold window chamber (DSFWC) model is described. A custom-made plastic window chamber structure was developed that is compatible with both imaging optical and MR imaging modalities and provides a model system for continuous study of the same tissue microenvironment on multiple imaging platforms over a 3-week period. For optical imaging of pH e, SNARF-1 carboxylic acid is injected intravenously into a SCID mouse with an implanted tumor. A ratiometric measurement of the fluorescence signal captured on a confocal microscope reveals the pHe of the tissue visible within the window chamber. This imaging method was used in a preliminary study to evaluate sodium bicarbonate as a potential drug treatment to reverse tissue acidosis. For MR imaging of pHe the chemical exchange saturation transfer (CEST) was used as an alternative way of measuring pHe in a DSFWC model. ULTRAVIST®, a FDA approved x-ray/CT contrast agent has been shown to have a CEST effect that is pH dependent. A ratiometric analysis of water saturation at 5.6 and 4.2 ppm chemical shift provides a means to estimate the local pHe.

AB - Upregulate levels of expression and activity of membrane H+ ion pumps in cancer cells drives the extracellular pH (pHe,) to values lower than normal. Furthermore, disregulated pH is indicative of the changes in glycolytic metabolism in tumor cells and has been shown to facilitate extracellular tissue remodeling during metastasis Therefore, measurement of pHe could be a useful cancer biomarker for diagnostic and therapy monitoring evaluation. Multimodality in-vivo imaging of pHe in tumorous tissue in a mouse dorsal skin fold window chamber (DSFWC) model is described. A custom-made plastic window chamber structure was developed that is compatible with both imaging optical and MR imaging modalities and provides a model system for continuous study of the same tissue microenvironment on multiple imaging platforms over a 3-week period. For optical imaging of pH e, SNARF-1 carboxylic acid is injected intravenously into a SCID mouse with an implanted tumor. A ratiometric measurement of the fluorescence signal captured on a confocal microscope reveals the pHe of the tissue visible within the window chamber. This imaging method was used in a preliminary study to evaluate sodium bicarbonate as a potential drug treatment to reverse tissue acidosis. For MR imaging of pHe the chemical exchange saturation transfer (CEST) was used as an alternative way of measuring pHe in a DSFWC model. ULTRAVIST®, a FDA approved x-ray/CT contrast agent has been shown to have a CEST effect that is pH dependent. A ratiometric analysis of water saturation at 5.6 and 4.2 ppm chemical shift provides a means to estimate the local pHe.

KW - acidosis

KW - cancer

KW - CEST

KW - confocal

KW - fluorescence

KW - in vivo imaging

KW - microenvironment

KW - MRI

KW - pH

KW - window chamber

UR - http://www.scopus.com/inward/record.url?scp=84877867004&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877867004&partnerID=8YFLogxK

U2 - 10.1117/12.2005472

DO - 10.1117/12.2005472

M3 - Conference contribution

AN - SCOPUS:84877867004

SN - 9780819493439

VL - 8574

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -