Multiphoton imaging of an in vitro ovarian tissue model

Nathaniel D. Kirkpatrick, Molly A. Brewer, Urs Utzinger

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In order to understand the distribution of endogenous fluorescence in the ovary, ovarian biopsies were maintained with a viable tissue imaging system and characterized with multiphoton imaging. It was imperative to maintain a stable in vitro environment so that tissue images could provide accurate correlative data for in vivo spectroscopic measurements. Evaluating tissue viability in real time poses a difficult task given that viability assays are tailored for cell culture. The focus of this study was to design a robust in vitro imaging chamber for assessment of ovarian autofluorescence and simple, reliable viability assays for tissue status monitoring.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsT. Wilson
Pages1-8
Number of pages8
Volume5860
StatePublished - 2005
EventConfocal, Multiphoton, and Nonlinear Microscopic Imaging II - Munich, Germany
Duration: Jun 12 2005Jun 16 2005

Other

OtherConfocal, Multiphoton, and Nonlinear Microscopic Imaging II
CountryGermany
CityMunich
Period6/12/056/16/05

Fingerprint

Tissue
Imaging techniques
Assays
Biopsy
Cell culture
Imaging systems
Fluorescence
Monitoring

Keywords

  • In vitro imaging
  • Multiphoton microscopy
  • Tissue diagnostics

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kirkpatrick, N. D., Brewer, M. A., & Utzinger, U. (2005). Multiphoton imaging of an in vitro ovarian tissue model. In T. Wilson (Ed.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5860, pp. 1-8). [586002]

Multiphoton imaging of an in vitro ovarian tissue model. / Kirkpatrick, Nathaniel D.; Brewer, Molly A.; Utzinger, Urs.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / T. Wilson. Vol. 5860 2005. p. 1-8 586002.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kirkpatrick, ND, Brewer, MA & Utzinger, U 2005, Multiphoton imaging of an in vitro ovarian tissue model. in T Wilson (ed.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5860, 586002, pp. 1-8, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, Munich, Germany, 6/12/05.
Kirkpatrick ND, Brewer MA, Utzinger U. Multiphoton imaging of an in vitro ovarian tissue model. In Wilson T, editor, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5860. 2005. p. 1-8. 586002
Kirkpatrick, Nathaniel D. ; Brewer, Molly A. ; Utzinger, Urs. / Multiphoton imaging of an in vitro ovarian tissue model. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / T. Wilson. Vol. 5860 2005. pp. 1-8
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