Multiphoton imaging of an in vitro ovarian tissue model

Nathaniel D. Kirkpatrick, Molly A. Brewer, Urs Utzinger

Research output: Contribution to journalConference article

Abstract

In order to understand the distribution of endogenous fluorescence in the ovary, ovarian biopsies were maintained with a viable tissue imaging system and characterized with multiphoton imaging. It was imperative to maintain a stable in vitro environment so that tissue images could provide accurate correlative data for in vivo spectroscopic measurements. Evaluating tissue viability in real time poses a difficult task given that viability assays are tailored for cell culture. The focus of this study was to design a robust in vitro imaging chamber for assessment of ovarian autofluorescence and simple, reliable viability assays for tissue status monitoring.

Original languageEnglish (US)
Article number586002
Pages (from-to)1-8
Number of pages8
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5860
StatePublished - Dec 19 2005
EventConfocal, Multiphoton, and Nonlinear Microscopic Imaging II - Munich, Germany
Duration: Jun 12 2005Jun 16 2005

Keywords

  • In vitro imaging
  • Multiphoton microscopy
  • Tissue diagnostics

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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