Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer

Christian F. Gainer, Marek Romanowski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

While upconverting lanthanide nanoparticles have numerous advantages over other exogenous contrast agents used in scanned multiphoton imaging, their long luminescence lifetimes cause images collected with non-descanned detection to be greatly blurred. We demonstrate herein the use of Richardson-Lucy deconvolution to deblur luminescence images obtained via multiphoton scanning microscopy. Images were taken of three dimensional models of colon and ovarian cancer following incubation with NaYF4:Yb,Er nanoparticles functionalized with an antibody for EGFR and folic acid respectively. Following deconvolution, images had a lateral resolution on par with the optimal performance of the imaging system used, ∼1.2 μm, and an axial resolution of ∼5 μm. Due to the relatively high multiphoton excitation efficiency of these nanoparticles, it is possible to follow binding of individual particles in tissue. In addition, their extreme photostability allows for prolonged imaging without significant loss in luminescence signal. With these advantageous properties in mind, we also discuss the potential application of upconverting lanthanide nanoparticles for tracking of specific, cancer relevant receptors in tissue.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8595
DOIs
StatePublished - 2013
EventColloidal Nanocrystals for Biomedical Applications VIII - San Francisco, CA, United States
Duration: Feb 2 2013Feb 4 2013

Other

OtherColloidal Nanocrystals for Biomedical Applications VIII
CountryUnited States
CitySan Francisco, CA
Period2/2/132/4/13

Fingerprint

Lanthanoid Series Elements
three dimensional models
Rare earth elements
Nanoparticles
Luminescence
cancer
Imaging techniques
nanoparticles
Deconvolution
luminescence
Neoplasms
folic acid
Tissue
antibodies
Folic Acid
Antibodies
Imaging systems
Ovarian Neoplasms
Colonic Neoplasms
Contrast Media

Keywords

  • colon cancer
  • EGFR
  • erbium
  • folate
  • lanthanide
  • multiphoton
  • nanoparticle
  • ovarian cancer

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Gainer, C. F., & Romanowski, M. (2013). Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8595). [85950O] https://doi.org/10.1117/12.2002551

Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer. / Gainer, Christian F.; Romanowski, Marek.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8595 2013. 85950O.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gainer, CF & Romanowski, M 2013, Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8595, 85950O, Colloidal Nanocrystals for Biomedical Applications VIII, San Francisco, CA, United States, 2/2/13. https://doi.org/10.1117/12.2002551
Gainer CF, Romanowski M. Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8595. 2013. 85950O https://doi.org/10.1117/12.2002551
Gainer, Christian F. ; Romanowski, Marek. / Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8595 2013.
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