Multiple continuous‐flow solid‐phase peptide synthesis Synthesis of an HIV antigenic peptide and its omission analogues

VIKTOR KRCHŇÁK, JOSEF VÁGNER, OTAKAR MACH

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Multiple continuous‐flow solid‐phase peptide synthesis was performed on a standard polystyrene‐based resin under low‐pressure conditions using a simple manually operated synthesizer. Stable‐flow resin‐packed columns were prepared in small polypropylene flow reactors, adjustable for volume. The concurrent synthesis of 10 peptides was carried out in flow reactors concatenated together; solvents and reactants were passed through this set of columns using moderate overpressure. One decapeptide, H‐Val‐Tyr‐Tyr‐Arg‐Asp‐Ser‐Arg‐Asn‐Pro‐Leu‐NH2, containing an antigenic determinant of the p31 protein product of the pol gene of the human immunodeficiency virus, and its nine omission analogues were synthesized.

Original languageEnglish (US)
Pages (from-to)209-213
Number of pages5
JournalInternational journal of peptide and protein research
Volume33
Issue number3
DOIs
StatePublished - Mar 1989

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Keywords

  • antigenic determinant
  • flow reactor
  • human immunodeficiency virus
  • multiple peptide synthesis
  • solid‐phase peptide synthesis

ASJC Scopus subject areas

  • Biochemistry

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