Multiple forms of rat kidney L-arginine: glycine amidinotransferase

M. D. Gross, Alex Simon, R. J. Jenny, E. D. Gray, D. M. McGuire, J. F. Van Pilsum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The relative amount of L-arginine:glycine amidinotransferase (transamidinase) protein in kidneys from rats fed a complete purified diet with and without the addition of creatine and/or glycine was determined by a monoclonal antibody-immunosorbent inhibition assay. Kidneys from the creatine-fed rats had 10% of the transamidinase activities 78% of the monoclonal antibody immunoreactive transamidinase protein as kidneys from the control rats. An excellent correlation between transamidinase activities and protein was reported previously when the amounts of enzyme protein were determined by immunotitration with polyclonal antibodies. One possible explanation for the contrasting results was that multiple forms of transamidinase are present in rat kidneys. If so, the monoclonal antibody may have recognized forms of the enzyme that were not decreased in amounts commensurate with the decrease in enzyme activities as a result of creatine feeding. Evidence is presented in this report that multiple forms of transamidinase are present in rat kidneys. The distribution of the isoelectric points of the individual forms of transamidinase in kidneys of the control rats appeared to be dissimilar from that in the creatine-fed rats. Therefore, an alteration in the distribution of the individual forms of the enzyme may be a factor in the alteration of transamidinase activities in creatine-fed rats.

Original languageEnglish (US)
Pages (from-to)1403-1409
Number of pages7
JournalJournal of Nutrition
Volume118
Issue number11
StatePublished - 1988
Externally publishedYes

Fingerprint

amidinotransferases
Amidinotransferases
glycine (amino acid)
arginine
creatine
kidneys
Creatine
Kidney
rats
monoclonal antibodies
Monoclonal Antibodies
Enzymes
Proteins
proteins
enzymes
Immunosorbents
glycine amidinotransferase
Isoelectric Point
isoelectric point
polyclonal antibodies

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Gross, M. D., Simon, A., Jenny, R. J., Gray, E. D., McGuire, D. M., & Van Pilsum, J. F. (1988). Multiple forms of rat kidney L-arginine: glycine amidinotransferase. Journal of Nutrition, 118(11), 1403-1409.

Multiple forms of rat kidney L-arginine : glycine amidinotransferase. / Gross, M. D.; Simon, Alex; Jenny, R. J.; Gray, E. D.; McGuire, D. M.; Van Pilsum, J. F.

In: Journal of Nutrition, Vol. 118, No. 11, 1988, p. 1403-1409.

Research output: Contribution to journalArticle

Gross, MD, Simon, A, Jenny, RJ, Gray, ED, McGuire, DM & Van Pilsum, JF 1988, 'Multiple forms of rat kidney L-arginine: glycine amidinotransferase', Journal of Nutrition, vol. 118, no. 11, pp. 1403-1409.
Gross MD, Simon A, Jenny RJ, Gray ED, McGuire DM, Van Pilsum JF. Multiple forms of rat kidney L-arginine: glycine amidinotransferase. Journal of Nutrition. 1988;118(11):1403-1409.
Gross, M. D. ; Simon, Alex ; Jenny, R. J. ; Gray, E. D. ; McGuire, D. M. ; Van Pilsum, J. F. / Multiple forms of rat kidney L-arginine : glycine amidinotransferase. In: Journal of Nutrition. 1988 ; Vol. 118, No. 11. pp. 1403-1409.
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