Multiple phases of competence occur during the Helicobacter pylori growth cycle

David A Baltrus, Karen Guillemin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The gastric pathogen Helicobacter pylori undergoes genetic exchange at unusually high frequencies, primarily through natural transformation. Despite progress toward understanding the molecular mechanism of natural transformation in H. pylori, little is known about how competence is regulated or its relationship to DNA release. By measuring transformation incrementally throughout the growth curve, we show that H. pylori exhibits a novel pattern of competence with distinct peaks of transformation during both logarithmic and stationary growth phases. Furthermore, different H. pylori strains vary in the presence and timing of their competence peaks. We also examined the process of DNA release in relation to competence. Although extensive DNA release does not occur until late stationary phase, sufficient genomic DNA was present during the logarithmic phase to yield measurable transformants. These results demonstrate that the state of competence in H. pylori occurs in an unprecedented pattern during the growth curve with no clear relationship to DNA release.

Original languageEnglish (US)
Pages (from-to)148-155
Number of pages8
JournalFEMS Microbiology Letters
Volume255
Issue number1
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Helicobacter pylori
Mental Competency
DNA
Growth
Stomach

Keywords

  • Competence
  • DNA release
  • Helicobacter pylori
  • Natural transformation
  • Physiology

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Multiple phases of competence occur during the Helicobacter pylori growth cycle. / Baltrus, David A; Guillemin, Karen.

In: FEMS Microbiology Letters, Vol. 255, No. 1, 02.2006, p. 148-155.

Research output: Contribution to journalArticle

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