Multiplexed 3D FRET imaging in deep tissue of live embryos

Ming Zhao, Xiaoyang Wan, Yu Li, Weibin Zhou, Leilei Peng

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of triple-channel intensity and lifetime images, we demonstrated that Ca 2+ and cAMP levels of live embryos expressing dual FRET sensors can be monitored simultaneously at microscopic resolution. The method is compatible with a broad range of FRET sensors currently available for probing various cellular biochemical functions. It opens the door to imaging complex cellular circuitries in whole live organisms.

Original languageEnglish (US)
Article number13991
JournalScientific reports
Volume5
DOIs
StatePublished - Sep 21 2015

ASJC Scopus subject areas

  • General

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