@inproceedings{2ce470f73483440cbda2e3f9f9bf3f5c,
title = "Multiplexed BioCD for prostate specific antigen detection",
abstract = "Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.",
keywords = "Antibody microarray, BioCD, Common-path, Label-free, Optical biosensor, PSA, Prostate cancer",
author = "Xuefeng Wang and Ming Zhao and Nolte, {David D.}",
year = "2008",
month = apr,
day = "21",
doi = "10.1117/12.759173",
language = "English (US)",
isbn = "9780819470232",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Advanced Biomedical and Clinical Diagnostic Systems VI",
note = "Advanced Biomedical and Clinical Diagnostic Systems VI ; Conference date: 20-01-2008 Through 21-01-2008",
}