Multiplexed BioCD for prostate specific antigen detection

Xuefeng Wang, Ming Zhao, David D. Nolte

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6848
DOIs
StatePublished - 2008
Externally publishedYes
EventAdvanced Biomedical and Clinical Diagnostic Systems VI - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems VI
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

Fingerprint

Antigens
Proteins
Antibodies
Body fluids
Biosensors
Statistical methods
Throughput

Keywords

  • Antibody microarray
  • BioCD
  • Common-path
  • Label-free
  • Optical biosensor
  • Prostate cancer
  • PSA

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wang, X., Zhao, M., & Nolte, D. D. (2008). Multiplexed BioCD for prostate specific antigen detection. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6848). [68480S] https://doi.org/10.1117/12.759173

Multiplexed BioCD for prostate specific antigen detection. / Wang, Xuefeng; Zhao, Ming; Nolte, David D.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6848 2008. 68480S.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, X, Zhao, M & Nolte, DD 2008, Multiplexed BioCD for prostate specific antigen detection. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6848, 68480S, Advanced Biomedical and Clinical Diagnostic Systems VI, San Jose, CA, United States, 1/20/08. https://doi.org/10.1117/12.759173
Wang X, Zhao M, Nolte DD. Multiplexed BioCD for prostate specific antigen detection. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6848. 2008. 68480S https://doi.org/10.1117/12.759173
Wang, Xuefeng ; Zhao, Ming ; Nolte, David D. / Multiplexed BioCD for prostate specific antigen detection. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6848 2008.
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