Multiplexed BioCD for prostate specific antigen detection

Xuefeng Wang, Ming Zhao, David D. Nolte

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VI
DOIs
StatePublished - Apr 21 2008
EventAdvanced Biomedical and Clinical Diagnostic Systems VI - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6848
ISSN (Print)1605-7422

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems VI
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

Keywords

  • Antibody microarray
  • BioCD
  • Common-path
  • Label-free
  • Optical biosensor
  • PSA
  • Prostate cancer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Wang, X., Zhao, M., & Nolte, D. D. (2008). Multiplexed BioCD for prostate specific antigen detection. In Advanced Biomedical and Clinical Diagnostic Systems VI [68480S] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6848). https://doi.org/10.1117/12.759173