Abstract
Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6848 |
| DOIs | |
| State | Published - 2008 |
| Externally published | Yes |
| Event | Advanced Biomedical and Clinical Diagnostic Systems VI - San Jose, CA, United States Duration: Jan 20 2008 → Jan 21 2008 |
Other
| Other | Advanced Biomedical and Clinical Diagnostic Systems VI |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/20/08 → 1/21/08 |
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Keywords
- Antibody microarray
- BioCD
- Common-path
- Label-free
- Optical biosensor
- Prostate cancer
- PSA
ASJC Scopus subject areas
- Engineering(all)
Cite this
Multiplexed BioCD for prostate specific antigen detection. / Wang, Xuefeng; Zhao, Ming; Nolte, David D.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6848 2008. 68480S.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Multiplexed BioCD for prostate specific antigen detection
AU - Wang, Xuefeng
AU - Zhao, Ming
AU - Nolte, David D.
PY - 2008
Y1 - 2008
N2 - Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.
AB - Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.
KW - Antibody microarray
KW - BioCD
KW - Common-path
KW - Label-free
KW - Optical biosensor
KW - Prostate cancer
KW - PSA
UR - http://www.scopus.com/inward/record.url?scp=42149114159&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149114159&partnerID=8YFLogxK
U2 - 10.1117/12.759173
DO - 10.1117/12.759173
M3 - Conference contribution
AN - SCOPUS:42149114159
SN - 9780819470232
VL - 6848
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -