Multiplication of Taura syndrome virus in primary hemocyte culture of shrimp (Penaeus vannamei)

Sunil K. George, Krista N. Kaizer, Yelena M. Betz, Arun K. Dhar

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The propagation of Taura syndrome virus (TSV) in primary hemocyte culture of Pacific white shrimp (Penaeus vannamei) was investigated. Purified TSV was inoculated into a 24. h old primary hemocyte culture and the development of cytopathic effects was monitored. The cell morphology started changing within 6. h post-inoculation; TSV-infected hemocytes started shrinking and granular structures began to form on the cell surface. There was a gradual loss of cell viability and, by 48. h post-inoculation, most cells detached from the bottom of the 96 well microplate. The propagation of TSV during the 48. h time course studied was measured by real-time RT-PCR. TSV copy number reached the highest level by 12. h post-inoculation and then started to decrease. Using an anti-TSV polyclonal antibody, the 55. kDa VP1 capsid protein was detected by Western blot analysis. The data suggest that shrimp primary hemocyte culture supports TSV replication and could be used as a tool for the study of host-virus interactions in TSV pathogenesis.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalJournal of Virological Methods
Volume172
Issue number1-2
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

Keywords

  • Hemocyte primary culture
  • Shrimp
  • TSV
  • Taura syndrome virus

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Multiplication of Taura syndrome virus in primary hemocyte culture of shrimp (Penaeus vannamei)'. Together they form a unique fingerprint.

  • Cite this