Multispectral fluorescence imaging of human ovarian and Fallopian tube tissue for early stage cancer detection

Tyler Tate, Brenda Baggett, Photini Rice, Jennifer Watson, Gabe Orsinger, Ariel C. Nymeyer, Weston A. Welge, Molly Keenan, Kathylynn Saboda, Denise Roe, Kenneth D Hatch, Setsuko K Chambers, John Black, Urs Utzinger, Jennifer K Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

With early detection, five year survival rates for ovarian cancer are over 90%, yet no effective early screening method exists. Emerging consensus suggests that perhaps over 50% of the most lethal form of the disease, high grade serous ovarian cancer, originates in the Fallopian tube. Cancer changes molecular concentrations of various endogenous fluorophores. Using specific excitation wavelengths and emissions bands on a Multispectral Fluorescence Imaging (MFI) system, spatial and spectral data over a wide field of view can be collected from endogenous fluorophores. Wavelength specific reflectance images provide additional information to normalize for tissue geometry and blood absorption. Ratiometric combination of the images may create high contrast between neighboring normal and abnormal tissue. Twenty-six women undergoing oophorectomy or debulking surgery consented the use of surgical discard tissue samples for MFI imaging. Forty-nine pieces of ovarian tissue and thirty-two pieces of Fallopian tube tissue were collected and imaged with excitation wavelengths between 280 nm and 550 nm. After imaging, each tissue sample was fixed, sectioned and HE stained for pathological evaluation. Comparison of mean intensity values between normal, benign, and cancerous tissue demonstrate a general trend of increased fluorescence of benign tissue and decreased fluorescence of cancerous tissue when compared to normal tissue. The predictive capabilities of the mean intensity measurements are tested using multinomial logistic regression and quadratic discriminant analysis. Adaption of the system for in vivo Fallopian tube and ovary endoscopic imaging is possible and is briefly described.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9313
ISBN (Print)9781628414035
DOIs
Publication statusPublished - 2015
EventAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII - San Francisco, United States
Duration: Feb 8 2015Feb 10 2015

Other

OtherAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII
CountryUnited States
CitySan Francisco
Period2/8/152/10/15

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Keywords

  • Auto-Fluorescence
  • Endogenous Fluorophores
  • Fallopian Tube
  • Hyperspectral Fluorescence Imaging
  • Multispectral Fluorescence Imaging
  • Ovarian Cancer
  • Ovary

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Tate, T., Baggett, B., Rice, P., Watson, J., Orsinger, G., Nymeyer, A. C., ... Barton, J. K. (2015). Multispectral fluorescence imaging of human ovarian and Fallopian tube tissue for early stage cancer detection. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9313). [93130L] SPIE. https://doi.org/10.1117/12.2079791