Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding

Diana Barritt, Henry I. Yamamura, William R. Roeske

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]c is methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2 nM, by 52%. Studies with several different ions demonstrated that the reduction in [3H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [3H](-) quinuclidinyl benzilate (QNB) was observed.

Original languageEnglish (US)
Pages (from-to)875-877
Number of pages3
JournalLife Sciences
Volume30
Issue number10
DOIs
StatePublished - Mar 8 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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