Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding

Diana Barritt, Henry I. Yamamura, William R Roeske

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]c is methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2 nM, by 52%. Studies with several different ions demonstrated that the reduction in [3H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [3H](-) quinuclidinyl benzilate (QNB) was observed.

Original languageEnglish (US)
Pages (from-to)875-877
Number of pages3
JournalLife Sciences
Volume30
Issue number10
DOIs
StatePublished - Mar 8 1982

Fingerprint

Muscarinic Receptors
Fluorides
Ions
Quinuclidinyl Benzilate
Ligands
Muscarinic Agonists
Sodium Fluoride
Muscarinic Antagonists
Modulation
Experiments

ASJC Scopus subject areas

  • Pharmacology

Cite this

Muscarinic receptor binding in the mouse heart : the selective modulatory effect of fluoride ion on agonist binding. / Barritt, Diana; Yamamura, Henry I.; Roeske, William R.

In: Life Sciences, Vol. 30, No. 10, 08.03.1982, p. 875-877.

Research output: Contribution to journalArticle

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