Muscle cell culture as a tool in animal growth research.

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Muscle cell culture techniques have been used for several years in research on muscle growth and development. Several types of culture systems have been devised, including primary cultures from embryonic or postnatal muscle and myogenic cell lines. In addition, serum-free and serum-containing media have been developed to address specific muscle development questions. Many of these questions center around muscle cell differentiation and muscle cell physiology; and, more recently, muscle cell cultures have been used as bioassay tools for examining growth physiology in domestic animals. In our laboratory, skeletal muscle satellite cells have been studied in vitro to evaluate the effect of several protein hormones and growth factors on satellite cell proliferation and differentiation. Of the hormones examined, only the insulin-like growth factors/somatomedins and fibroblast growth factor have been shown to have a stimulatory effect on proliferation that could be physiologically significant. None of the major anterior pituitary hormones interacted directly with satellite cells to stimulate proliferation. With advances in serum-free medium formulations and cell separation techniques, more information can be obtained from experiments with muscle cell cultures. With appropriate design and interpretation, our knowledge of muscle growth in domestic animals will be expanded.

Original languageEnglish (US)
Pages (from-to)290-294
Number of pages5
JournalFederation Proceedings
Volume46
Issue number2
StatePublished - Feb 1987
Externally publishedYes

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Muscle Cells
Cell Culture Techniques
Growth
Muscle Development
Serum-Free Culture Media
Domestic Animals
Somatomedins
Cell Differentiation
Skeletal Muscle Satellite Cells
Hormones
Anterior Pituitary Hormones
Cell Physiological Phenomena
Fibroblast Growth Factors
Cell Separation
Growth and Development
Biological Assay
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Cell Line
Muscles

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Muscle cell culture as a tool in animal growth research. / Allen, Ronald E.

In: Federation Proceedings, Vol. 46, No. 2, 02.1987, p. 290-294.

Research output: Contribution to journalArticle

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