Mutants in Arabidopsis thaliana altered in epicuticular wax and leaf morphology

Matthew A. Jenks, Aaron M. Rashotte, Hillary A. Tuttle, Kenneth A. Feldmann

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

We report eight new mutants in Arabidopsis thaliana possessing altered leaf morphology and epicuticular wax. These were isolated from a T-DNA-mutagenized population using a visual screen for altered leaf reflectance, i.e. increased glaucousness or glossiness. The mutants were placed into three distinct classes based on alterations in overall plant morphology: knobhead (knb), bicentifolia (bcf), and wax. The four knb mutants formed callus-like growths in the axillary region of the rosette leaves and apical meristem, the two bcf mutants produced hundreds of narrow leaves, and the two wax mutants had leaves and stems that were more glossy than wild type and organs that fused during early development. Leaves of knb and bcf were more glaucous and abnormally shaped than wild type. Epicuticular wax crystals over knb and bcf leaf surfaces (where none were present on wild type) likely contributed to their more glaucous appearance. In contrast, the glossy appearance of the wax mutants was associated with a reduced epicuticular wax load on both leaves and stems. One representative from each phenotypic class was selected for detailed analyses of epicuticular wax chemistry. All three lines, knb1, bcf1, and wax1, had dramatic alterations in the total amounts and relative proportions of their leaf epicuticular wax constituents.

Original languageEnglish (US)
Pages (from-to)377-385
Number of pages9
JournalPlant physiology
Volume110
Issue number2
DOIs
StatePublished - Feb 1996

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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