Myoblast Stored Myosin Heavy Chain Transcripts are Precursors to the Myotube Polysomal Myosin Heavy Chain mRNAs

THOMAS C. DOETSCHMAN, HAVIV P. DYM, ERIC J. SIEGEL, STUART M. HEYWOOD

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Radioactively labeled myosin heavy chain messenger ribonucleic acid (MHC mRNA) synthesized during the pre-fusion stage of chick embryo breast muscle cell culture is transferred from messenger ribonucleic acid proteins (mRNPs) to the polysomal MHC mRNA during the period of rapid increase in the rate of MHC synthesis (mid-to late-fusion). This transfer constitutes a major contribution to the rate of incorporation of 3H-labeled transcripts into polysomal MHC mRNA at this time. As the increase in the rate of MHC synthesis levels off (late-to post-fusion) the contribution to the rate of incorporation of 3H-labeled transcripts into polysomal MHC mRNA from newly synthesized transcripts increases until it becomes predominant. In vivo, the level of MHC mRNP increases during early stages of embryonic development and then decreases when MHC synthesis and the level of polysomal MHC mRNA has been shown to increase.

Original languageEnglish (US)
Pages (from-to)149-162
Number of pages14
JournalDifferentiation
Volume16
Issue number1-3
DOIs
StatePublished - 1980

Keywords

  • HP
  • MHC
  • PBS
  • heavy polysomes
  • mRNP
  • messenger ribonucleic acid proteins
  • myosin heavy chain
  • phosphate buffered saline

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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