Myocardin expression during avian embryonic heart development requires the endoderm but is independent of BMP signaling

Andrew S. Warkman, Tatiana A. Yatskievych, Katharine M. Hardy, Paul A Krieg, Parker B Antin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Myocardin, a serum response factor cofactor, plays an important role in regulating heart and smooth muscle development. To investigate myocardin function during early stages of heart development, we isolated the chicken orthologue of myocardin and characterized its expression between Hamburger and Hamilton stages 3 and 15. At stage 4, myocardin transcripts are detected in the lateral and extraembryonic mesoderm, become progressively localized to the precardiac mesoderm and the differentiated myocardium and are also seen in smooth muscle cells of the developing vascular plexus. Surprisingly, myocardin expression within the developing chicken embryo precedes that of the homeodomain transcription factor Nkx2.5. Embryonic dissection studies demonstrate that signals from the endoderm are required for myocardin expression within the precardiac mesoderm. However, unlike Nkx2.5, myocardin expression is not regulated by bone morphogenetic protein (BMP) signaling. These results suggest that initial expression of myocardin in the precardiac mesoderm is regulated by a signaling pathway that is parallel to, and independent of, Nkx2.5 expression.

Original languageEnglish (US)
Pages (from-to)216-221
Number of pages6
JournalDevelopmental Dynamics
Volume237
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Endoderm
Bone Morphogenetic Proteins
Embryonic Development
Mesoderm
Chickens
Myocardium
Serum Response Factor
myocardin
Muscle Development
Smooth Muscle Myocytes
Smooth Muscle
Blood Vessels
Dissection
Transcription Factors
Embryonic Structures

Keywords

  • Bmp signaling
  • Cardiac myogenesis
  • Gene regulation
  • Myocardin
  • Noggin
  • Precardiac mesoderm

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Myocardin expression during avian embryonic heart development requires the endoderm but is independent of BMP signaling. / Warkman, Andrew S.; Yatskievych, Tatiana A.; Hardy, Katharine M.; Krieg, Paul A; Antin, Parker B.

In: Developmental Dynamics, Vol. 237, No. 1, 01.2008, p. 216-221.

Research output: Contribution to journalArticle

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