Myocilin-associated exosomes in human ocular samples

K. M. Perkumas, E. A. Hoffman, Brian S Mckay, R. R. Allingham, W. D. Stamer

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Mutations in myocilin result in ocular hypertension, likely due to decreased drainage of aqueous humor through the trabecular meshwork. Since less myocilin is found in the aqueous humor of those with disease-causing mutations, understanding myocilin's role in the aqueous humor is of clinical importance. Recently, myocilin was shown to exit cultured trabecular meshwork cells in association with shed vesicles called exosomes. To examine relevance of this finding in a physiological setting, the present study examined three different types of ocular samples for the presence of myocilin-associated exosomes. Using differential centrifugation steps, we found myocilin associated with exosomes isolated from effluent collected from human anterior segments in organ culture and aqueous humor obtained from human cadaveric eyes or from patients undergoing excisional surgery. Similar to results with cultured cells, myocilin associated predominately with exosomes in fresh samples, appeared mostly soluble at later times, and had biochemical properties (density of 1.13-1.19 g/ml in linear sucrose gradient) similar to those characteristics of exosomes. These data indicate that exosomes are present and may facilitate the transport of myocilin into the extracellular space of human ocular cells.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalExperimental Eye Research
Volume84
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Exosomes
Aqueous Humor
Trabecular Meshwork
Ocular Hypertension
Mutation
trabecular meshwork-induced glucocorticoid response protein
Organ Culture Techniques
Extracellular Space
Centrifugation
Sucrose
Drainage
Cultured Cells

Keywords

  • aqueous humor
  • cell culture
  • glaucoma
  • organ culture
  • trabecular meshwork

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Perkumas, K. M., Hoffman, E. A., Mckay, B. S., Allingham, R. R., & Stamer, W. D. (2007). Myocilin-associated exosomes in human ocular samples. Experimental Eye Research, 84(1), 209-212. https://doi.org/10.1016/j.exer.2006.09.020

Myocilin-associated exosomes in human ocular samples. / Perkumas, K. M.; Hoffman, E. A.; Mckay, Brian S; Allingham, R. R.; Stamer, W. D.

In: Experimental Eye Research, Vol. 84, No. 1, 01.2007, p. 209-212.

Research output: Contribution to journalArticle

Perkumas, KM, Hoffman, EA, Mckay, BS, Allingham, RR & Stamer, WD 2007, 'Myocilin-associated exosomes in human ocular samples', Experimental Eye Research, vol. 84, no. 1, pp. 209-212. https://doi.org/10.1016/j.exer.2006.09.020
Perkumas, K. M. ; Hoffman, E. A. ; Mckay, Brian S ; Allingham, R. R. ; Stamer, W. D. / Myocilin-associated exosomes in human ocular samples. In: Experimental Eye Research. 2007 ; Vol. 84, No. 1. pp. 209-212.
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