Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy

Honghai Liu, Yonghong Shao, Wan Qin, Raymond B Runyan, Meifeng Xu, Zhen Ma, Thomas K. Borg, Roger Markwald, Bruce Z. Gao

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.

Original languageEnglish (US)
Pages (from-to)262-270
Number of pages9
JournalCardiovascular Research
Volume97
Issue number2
DOIs
StatePublished - Feb 1 2013

Fingerprint

Myofibrils
Myosins
Cardiac Myocytes
Muscle Development
Microscopy
Sarcomeres
Incubators
Photons
Myocardium
Fluorescence

Keywords

  • Myofibrillogenesis
  • Myosin filaments
  • On-stage incubator
  • Sarcomere addition
  • Second harmonic generation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Physiology

Cite this

Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy. / Liu, Honghai; Shao, Yonghong; Qin, Wan; Runyan, Raymond B; Xu, Meifeng; Ma, Zhen; Borg, Thomas K.; Markwald, Roger; Gao, Bruce Z.

In: Cardiovascular Research, Vol. 97, No. 2, 01.02.2013, p. 262-270.

Research output: Contribution to journalArticle

Liu, Honghai ; Shao, Yonghong ; Qin, Wan ; Runyan, Raymond B ; Xu, Meifeng ; Ma, Zhen ; Borg, Thomas K. ; Markwald, Roger ; Gao, Bruce Z. / Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy. In: Cardiovascular Research. 2013 ; Vol. 97, No. 2. pp. 262-270.
@article{b24b2718bff0436188f8fccadda4237e,
title = "Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy",
abstract = "AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.",
keywords = "Myofibrillogenesis, Myosin filaments, On-stage incubator, Sarcomere addition, Second harmonic generation",
author = "Honghai Liu and Yonghong Shao and Wan Qin and Runyan, {Raymond B} and Meifeng Xu and Zhen Ma and Borg, {Thomas K.} and Roger Markwald and Gao, {Bruce Z.}",
year = "2013",
month = "2",
day = "1",
doi = "10.1093/cvr/cvs328",
language = "English (US)",
volume = "97",
pages = "262--270",
journal = "Cardiovascular Research",
issn = "0008-6363",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy

AU - Liu, Honghai

AU - Shao, Yonghong

AU - Qin, Wan

AU - Runyan, Raymond B

AU - Xu, Meifeng

AU - Ma, Zhen

AU - Borg, Thomas K.

AU - Markwald, Roger

AU - Gao, Bruce Z.

PY - 2013/2/1

Y1 - 2013/2/1

N2 - AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.

AB - AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.

KW - Myofibrillogenesis

KW - Myosin filaments

KW - On-stage incubator

KW - Sarcomere addition

KW - Second harmonic generation

UR - http://www.scopus.com/inward/record.url?scp=84872547219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872547219&partnerID=8YFLogxK

U2 - 10.1093/cvr/cvs328

DO - 10.1093/cvr/cvs328

M3 - Article

C2 - 23118131

AN - SCOPUS:84872547219

VL - 97

SP - 262

EP - 270

JO - Cardiovascular Research

JF - Cardiovascular Research

SN - 0008-6363

IS - 2

ER -