N-methyl-D-aspartate receptor binding is altered and seizure potential reduced in pregnant rats

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The objective of this study was to determine if a change in brain tissue excitatory amino acid receptor binding occurs during pregnancy using in vitro quantitative autoradiography and to examine seizure potential during pregnancy via central injection of N-methyl-D-aspartate (NMDA). For the receptor autoradiography studies, eight pregnant rats (day 21) and eight non-pregnant rats were euthanized with carbon dioxide, perfused, their brains dissected and frozen. Cryostat sections were taken and labeled in vitro by one of the following ligands: [3H]-CGP 39653, [3H]-glycine, [3H]-MK-801, [3H]-2-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) or [3H]-kainate. Optical density measurements of binding in 11 brain regions were performed using image analysis. To test seizure susceptibility, 74 rats were surgically implanted with an electrode into the hippocampus and a cannula into the lateral ventricle. Rats were mated; others served as non-pregnant controls. On gestational day 20, rats were randomized to receive no drug or an injection of NMDA (34, 68 or 136 nmol) through their indwelling cannulae. Seizures were assessed for 20 min. During pregnancy, the density of the NMDA competitive antagonist site measured by [3H]-CGP 39653 was decreased in the hippocampus, thalamus and hypothalamus (P<0.01), while the glycine modulation site was decreased in the cortex, hippocampus, thalamus, caudate and cerebellum (P<0.01). Kainate binding was significantly decreased in the hippocampus (P<0.05). Total seizure duration and total number of seizures were significantly reduced in pregnant vs. non-pregnant rats (P<0.05). Pregnancy is associated with a significant alteration of NMDA and non-NMDA receptor binding in rats. These findings suggest that pregnancy affords some protection against seizures induced by an activation of NMDA receptors in the brain. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)10-19
Number of pages10
JournalBrain Research
Volume844
Issue number1-2
DOIs
StatePublished - Oct 9 1999
Externally publishedYes

Fingerprint

N-Methyl-D-Aspartate Receptors
Seizures
N-Methylaspartate
Hippocampus
Pregnancy
Kainic Acid
Brain
Autoradiography
Thalamus
Glycine
Isoxazoles
D-Aspartic Acid
Implanted Electrodes
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Injections
Dizocilpine Maleate
Lateral Ventricles
Propionates
Glutamate Receptors
Carbon Dioxide

Keywords

  • Epilepsy
  • Glutamate
  • NMDA
  • Pregnancy
  • Rat
  • Seizure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

N-methyl-D-aspartate receptor binding is altered and seizure potential reduced in pregnant rats. / Standley, Cynthia A.

In: Brain Research, Vol. 844, No. 1-2, 09.10.1999, p. 10-19.

Research output: Contribution to journalArticle

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