Naive CD8+ T cells do not require costimulation for proliferation and differentiation into cytotoxic effector cells

Bo Wang, Robert Maile, Roberta Greenwood, Edward J. Collins, Jeffrey A Frelinger

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Most current models of T cell activation postulate a requirement for two distinct signals. One signal is delivered through the TCR by engagement with peptide/MHC complexes, and the second is delivered by interaction between costimulatory molecules such as CD28 and its ligands CD80 and CD86. Soluble peptide/MHC tetramers provide an opportunity to test whether naive CD8+ T cells can be activated via the signal generated through the TCR-αβ in the absence of any potential costimulatory molecules. Using T cells from two different TCR transgenic mice in vitro, we find that TCR engagement by peptide/MHC tetramers is sufficient for the activation of naive CD8+ T cells. Furthermore, these T cells proliferate, produce cytokines, and differentiate into cytolytic effectors. Under the conditions where anti-CD28 is able to enhance proliferation of normal B6 CD4+, CD8+, and TCR transgenic CD8+ T cells with anti-CD3, we see no effect of anti-CD28 on proliferation induced by tetramers. The results of this experiment argue that given a strong signal delivered through the TCR by an authentic ligand, no costimulation is required.

Original languageEnglish (US)
Pages (from-to)1216-1222
Number of pages7
JournalJournal of Immunology
Volume164
Issue number3
StatePublished - Feb 1 2000
Externally publishedYes

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T-Lymphocytes
Peptides
Ligands
Transgenic Mice
Cytokines

ASJC Scopus subject areas

  • Immunology

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Naive CD8+ T cells do not require costimulation for proliferation and differentiation into cytotoxic effector cells. / Wang, Bo; Maile, Robert; Greenwood, Roberta; Collins, Edward J.; Frelinger, Jeffrey A.

In: Journal of Immunology, Vol. 164, No. 3, 01.02.2000, p. 1216-1222.

Research output: Contribution to journalArticle

Wang, Bo ; Maile, Robert ; Greenwood, Roberta ; Collins, Edward J. ; Frelinger, Jeffrey A. / Naive CD8+ T cells do not require costimulation for proliferation and differentiation into cytotoxic effector cells. In: Journal of Immunology. 2000 ; Vol. 164, No. 3. pp. 1216-1222.
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