Nanotome Cluster Bombardment to Recover Spatial Chemistry After Preparation of Biological Samples for SIMS Imaging

Michael E. Kurczy, Paul D. Piehowsky, David Willingham, Kathleen A. Molyneaux, Michael L Heien, Nicholas Winograd, Andrew G. Ewing

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A C60+ cluster ion projectile is employed for sputter cleaning biological surfaces to reveal spatio-chemical information obscured by contamination overlayers. This protocol is used as a supplemental sample preparation method for time of flight secondary ion mass spectrometry (ToF-SIMS) imaging of frozen and freeze-dried biological materials. Following the removal of nanometers of material from the surface using sputter cleaning, a frozen-patterned cholesterol film and a freeze-dried tissue sample were analyzed using ToF-SIMS imaging. In both experiments, the chemical information was maintained after the sputter dose, due to the minimal chemical damage caused by C60+ bombardment. The damage to the surface produced by freeze-drying the tissue sample was found to have a greater effect on the loss of cholesterol signal than the sputter-induced damage. In addition to maintaining the chemical information, sputtering is not found to alter the spatial distribution of molecules on the surface. This approach removes artifacts that might obscure the surface chemistry of the sample and are common to many biological sample preparation schemes for ToF-SIMS imaging.

Original languageEnglish (US)
Pages (from-to)833-836
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Volume21
Issue number5
DOIs
StatePublished - May 2010
Externally publishedYes

Fingerprint

Secondary Ion Mass Spectrometry
Secondary ion mass spectrometry
Imaging techniques
Cholesterol
Cleaning
Freeze Drying
Tissue
Artifacts
Projectiles
Surface chemistry
Biological materials
Ions
Spatial distribution
Sputtering
Drying
Contamination
Molecules
Experiments

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

Nanotome Cluster Bombardment to Recover Spatial Chemistry After Preparation of Biological Samples for SIMS Imaging. / Kurczy, Michael E.; Piehowsky, Paul D.; Willingham, David; Molyneaux, Kathleen A.; Heien, Michael L; Winograd, Nicholas; Ewing, Andrew G.

In: Journal of the American Society for Mass Spectrometry, Vol. 21, No. 5, 05.2010, p. 833-836.

Research output: Contribution to journalArticle

Kurczy, Michael E. ; Piehowsky, Paul D. ; Willingham, David ; Molyneaux, Kathleen A. ; Heien, Michael L ; Winograd, Nicholas ; Ewing, Andrew G. / Nanotome Cluster Bombardment to Recover Spatial Chemistry After Preparation of Biological Samples for SIMS Imaging. In: Journal of the American Society for Mass Spectrometry. 2010 ; Vol. 21, No. 5. pp. 833-836.
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