Natriuretic and depolarizing effects of a stable fly (Stomoxys calcitrans) factor on Malpighian tubules

Andrew C. Chen, Thomas L Pannabecker, Demar Taylor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A two-step HPLC purification procedure resulted in a factor from the stable fly that depolarizes the lumen-negative transepithelial voltage (V(t)) of the adult stable fly Malpighian tubule. When applied to tubules of the female mosquito, Aedes aegypti, this factor partially mimics the electrophysiological actions of the mosquito natriuretic factor (MNF). It also selectively increases active transepithelial Na transport by the mosquito Malpighian tubule. The blood meal causes a transient increase in hemolymph Na and Cl contents and hemolymph volume during the course of the 24-h post-feeding period. The level of a factor that is immunologically cross-reactive with the human atrial natriuretic peptide (ANP) increases more than 6-fold within 6 h following a blood meal by the stable fly. The temporal pattern of the levels of the ANP-immunoreactive factor closely parallels the blood meal-induced rise and subsequent fall in hemolymph NaCl content and hemolymph volume, suggesting a functional correlation between the ANP-immunoreactive factor and the rate of NaCl and fluid loss from the hemolymph.

Original languageEnglish (US)
Pages (from-to)991-998
Number of pages8
JournalJournal of Insect Physiology
Volume43
Issue number11
DOIs
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

Muscidae
Malpighian Tubules
Natriuretic Agents
Stomoxys calcitrans
Malpighian tubules
Hemolymph
hemolymph
atrial natriuretic peptide
blood meal
Atrial Natriuretic Factor
Culicidae
Meals
Aedes
Aedes aegypti
High Pressure Liquid Chromatography

Keywords

  • Aedes aegypti
  • Diuretic hormone
  • Excretion
  • Ion transport
  • Stomoxys calcitrans

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Natriuretic and depolarizing effects of a stable fly (Stomoxys calcitrans) factor on Malpighian tubules. / Chen, Andrew C.; Pannabecker, Thomas L; Taylor, Demar.

In: Journal of Insect Physiology, Vol. 43, No. 11, 10.1997, p. 991-998.

Research output: Contribution to journalArticle

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AB - A two-step HPLC purification procedure resulted in a factor from the stable fly that depolarizes the lumen-negative transepithelial voltage (V(t)) of the adult stable fly Malpighian tubule. When applied to tubules of the female mosquito, Aedes aegypti, this factor partially mimics the electrophysiological actions of the mosquito natriuretic factor (MNF). It also selectively increases active transepithelial Na transport by the mosquito Malpighian tubule. The blood meal causes a transient increase in hemolymph Na and Cl contents and hemolymph volume during the course of the 24-h post-feeding period. The level of a factor that is immunologically cross-reactive with the human atrial natriuretic peptide (ANP) increases more than 6-fold within 6 h following a blood meal by the stable fly. The temporal pattern of the levels of the ANP-immunoreactive factor closely parallels the blood meal-induced rise and subsequent fall in hemolymph NaCl content and hemolymph volume, suggesting a functional correlation between the ANP-immunoreactive factor and the rate of NaCl and fluid loss from the hemolymph.

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