Natural killer cells play a key role in the antitumor immunity generated by chaperone-rich cell lysate vaccination

Yi - Zeng, Xinchun Chen, Nicolas Larmonier, Claire B Larmonier, Gang Li, Marjan Sepassi, Marilyn Marron, Samita Andreansky, Emmanuel Katsanis

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Tumor derived chaperone-rich cell lysate (CRCL) when isolated from tumor tissues is a potent vaccine that contains at least 4 of the highly immunogenic heat shock proteins (HSP) such as HSP70, HSP90, glucose related protein 94 and calreticulin. We have previously documented that CRCL provides both a source of tumor antigens and danger signals triggering dendritic cell (DC) activation. Immunization with tumor derived CRCL elicits tumor-specific T cell responses leading to tumor regression. In the current study, we further dissect the mechanisms by which CRCL simulates the immune system, and demonstrate that natural killer (NK) cells are required for effective antitumor effects to take place. Our results illustrate that CRCL directly stimulates proinflammatory cytokine and chemokine production by NK cells, which may lead to activation and recruitment of macrophages at the tumor site. Thus, this report provides further insight into the function of CRCL as an immunostimulant against cancer.

Original languageEnglish (US)
Pages (from-to)2624-2631
Number of pages8
JournalInternational Journal of Cancer
Volume119
Issue number11
DOIs
StatePublished - Dec 1 2006

Fingerprint

Natural Killer Cells
Immunity
Vaccination
Neoplasms
Calreticulin
Immunologic Adjuvants
Macrophage Activation
Neoplasm Antigens
Heat-Shock Proteins
Chemokines
Dendritic Cells
Immune System
Immunization
Vaccines
Cytokines
T-Lymphocytes
Glucose
Proteins

Keywords

  • Chaperone/heat shock proteins
  • CML
  • NK cell

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Natural killer cells play a key role in the antitumor immunity generated by chaperone-rich cell lysate vaccination. / Zeng, Yi -; Chen, Xinchun; Larmonier, Nicolas; Larmonier, Claire B; Li, Gang; Sepassi, Marjan; Marron, Marilyn; Andreansky, Samita; Katsanis, Emmanuel.

In: International Journal of Cancer, Vol. 119, No. 11, 01.12.2006, p. 2624-2631.

Research output: Contribution to journalArticle

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AU - Li, Gang

AU - Sepassi, Marjan

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AU - Andreansky, Samita

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