Natural thermal resistance of human tumor cells and the role of prostaglandin

F. Kristian Storm, Denise Roe, Susanne Hildebrand-Zanki, Gloria D. Worth, Donald L. Morton, David H. Kern

Research output: Contribution to journalArticle

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Abstract

We found that human malignant melanoma cells had varying thermal sensitivity and that some exhibited natural thermal resistance, a heretofore unrecognized phenomenon. Samples of 73 melanoma cell suspensions were heated at 42 °C for 1 hour before plating in the soft agar clonogenic assay or the thymidine assay for proliferating cells. We observed greater than 75% cell kill after hyperthermia in 39 (53%) tumors. Native thermal resistance was apparent in 17 (23%) tumors and growth enhancement in 17 (23%) at this temperature and exposure time. We postulated that prostaglandin, known to protect stomach mucosa against thermal injury, has a role in stabilizing the tumor cell membrane exposed to heat. Three melanoma cell lines known to be thermosensitive were heated to 42 °C without and with exogenous prostaglandin E2 (PGE2). The survival of colony-forming cells was increased in all three lines in the presence of 30 μM PGE2. A naturally thermoresistant cell line was exposed to 1 μg/ml indomethacin for 24 hours before hyperthermic treatment. The survival of colony-forming cells was significantly decreased compared to cells not treated with indomethacin. The addition of 30 μM of exogenous PGE2 to indomethacin-treated cells reestablished thermal resistance. These preliminary data suggest that some tumor cells synthesize prostaglandins to render the cell thermoresistant. Treatment with indomethacin blocks prostaglandin synthesis and induces thermosensitivity. These discoveries may have important clinical applications for hyperthermia treatment of human cancers.

Original languageEnglish (US)
Pages (from-to)376-383
Number of pages8
JournalSurgery
Volume94
Issue number2
StatePublished - 1983
Externally publishedYes

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Innate Immunity
Prostaglandins
Hot Temperature
Neoplasms
Indomethacin
Dinoprostone
Melanoma
Fever
Cell Line
Thymidine
Agar
Stomach
Suspensions
Mucous Membrane
Cell Membrane
Temperature
Wounds and Injuries
Growth

ASJC Scopus subject areas

  • Surgery

Cite this

Storm, F. K., Roe, D., Hildebrand-Zanki, S., Worth, G. D., Morton, D. L., & Kern, D. H. (1983). Natural thermal resistance of human tumor cells and the role of prostaglandin. Surgery, 94(2), 376-383.

Natural thermal resistance of human tumor cells and the role of prostaglandin. / Storm, F. Kristian; Roe, Denise; Hildebrand-Zanki, Susanne; Worth, Gloria D.; Morton, Donald L.; Kern, David H.

In: Surgery, Vol. 94, No. 2, 1983, p. 376-383.

Research output: Contribution to journalArticle

Storm, FK, Roe, D, Hildebrand-Zanki, S, Worth, GD, Morton, DL & Kern, DH 1983, 'Natural thermal resistance of human tumor cells and the role of prostaglandin', Surgery, vol. 94, no. 2, pp. 376-383.
Storm FK, Roe D, Hildebrand-Zanki S, Worth GD, Morton DL, Kern DH. Natural thermal resistance of human tumor cells and the role of prostaglandin. Surgery. 1983;94(2):376-383.
Storm, F. Kristian ; Roe, Denise ; Hildebrand-Zanki, Susanne ; Worth, Gloria D. ; Morton, Donald L. ; Kern, David H. / Natural thermal resistance of human tumor cells and the role of prostaglandin. In: Surgery. 1983 ; Vol. 94, No. 2. pp. 376-383.
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