Near-infrared Raman spectroscopy for in vivo detection of cervical precancers

U. Utzinger, D. L. Heintzelman, A. Mahadevan-Jansen, A. Malpica, M. Follen, R. Richards-Kortum

Research output: Contribution to journalArticle

214 Scopus citations

Abstract

This study evaluates the potential of near-infrared Raman spectroscopy for in vivo detection of squamous dysplasia, a precursor to cervical cancer. A pilot clinical trial was carried out at three clinical sites. Raman spectra were measured from one colposcopically normal and one abnormal area of the cervix. These sites were then biopsied and submitted for routine histologic analysis. Twenty-four evaluable measurements were made in vivo in 13 patients. Cervical tissue Raman spectra contain peaks in the vicinity of 1070, 1180, 1195, 1210, 1245, 1330, 1400, 1454, 1505, 1555, 1656, and 1760 cm-1. The ratio of intensities at 1454 to 1656 cm-1 is greater for squamous dysplasia than all other tissue types, while the ratio of intensities at 1330 to 1454 cm-1 is lower for samples with squamous dysplasia than all other tissue types. A simple algorithm based on these two intensity ratios separates high-grade squamous dysplasia from all others, misclassifying only one sample. Spectra measured in vivo resemble those measured in vitro. Cervical epithelial cells may contribute to tissue spectra at 1330 cm-1, a region associated with DNA. In contrast, epithelial cells probably do not contribute to tissue spectra at 1454 cm-1, a region associated with collagen and phospholipids.

Original languageEnglish (US)
Pages (from-to)955-959
Number of pages5
JournalApplied Spectroscopy
Volume55
Issue number8
DOIs
StatePublished - Aug 1 2001
Externally publishedYes

Keywords

  • Cervix
  • In vivo study
  • Raman spectroscopy

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

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  • Cite this

    Utzinger, U., Heintzelman, D. L., Mahadevan-Jansen, A., Malpica, A., Follen, M., & Richards-Kortum, R. (2001). Near-infrared Raman spectroscopy for in vivo detection of cervical precancers. Applied Spectroscopy, 55(8), 955-959. https://doi.org/10.1366/0003702011953018