Near real-time detection of E. coli in reclaimed water

Samendra Sherchan, Syreeta Miles, Luisa Ikner, Hye Weon Yu, Shane A Snyder, Ian L Pepper

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Advanced treatment of reclaimed water prior to potable reuse normally results in the inactivation of bacterial populations, however, incremental treatment failure can result in bacteria, including pathogens, remaining viable. Therefore, potential microorganisms need to be detected in real-time to preclude potential adverse human health effects. Real-time detection of microbes presents unique problems which are dependent on the water quality of the test water, including parameters such as particulate content and turbidity, and natural organic matter content. In addition, microbes are unusual in that: (i) viability and culturability are not always synonymous; (ii) viability in water can be reduced by osmotic stress; and (iii) bacteria can invoke repair mechanisms in response to UV disinfection resulting in regrowth of bacterial populations. All these issues related to bacteria affect the efficacy of real-time detection for bacteria. Here we evaluate three different sensors suitable for specific water qualities. The sensor A is an on-line, real-time sensor that allows for the continuous monitoring of particulates (including microbial contaminants) using multi-angle-light scattering (MALS) technology. The sensor B is a microbial detection system that uses optical technique, Mie light scattering, for particle sizing and fluorescence emission for viable bacteria detection. The last sensor C was based on adenosine triphosphate (ATP) production. E. coli was used a model organism and out of all tested sensors, we found the sensor C to be the most accurate. It has a great potential as a surrogate parameter for microbial loads in test waters and be useful for process control in treatment trains.

Original languageEnglish (US)
Article number2303
JournalSensors (Switzerland)
Volume18
Issue number7
DOIs
StatePublished - Jul 16 2018

Fingerprint

Escherichia coli
Bacteria
bacteria
Water
sensors
Sensors
water
microorganisms
Water Quality
water quality
viability
Light scattering
particulates
Light
Water quality
Optical Devices
Water Purification
light scattering
Disinfection
Osmotic Pressure

Keywords

  • Microorganism
  • Monitoring
  • Online sensors
  • Water quality
  • Water reuse
  • Water supply

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Near real-time detection of E. coli in reclaimed water. / Sherchan, Samendra; Miles, Syreeta; Ikner, Luisa; Yu, Hye Weon; Snyder, Shane A; Pepper, Ian L.

In: Sensors (Switzerland), Vol. 18, No. 7, 2303, 16.07.2018.

Research output: Contribution to journalArticle

Sherchan, Samendra ; Miles, Syreeta ; Ikner, Luisa ; Yu, Hye Weon ; Snyder, Shane A ; Pepper, Ian L. / Near real-time detection of E. coli in reclaimed water. In: Sensors (Switzerland). 2018 ; Vol. 18, No. 7.
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