Nedocromil Sodium Inhibits Canine Adenovirus Bronchiolitis in Beagle Puppies

Kurt A. Anderson, Richard J. Lemen, Natalie S. Weger, Natalie S. Huizhong Chen, Mark C. Bowers, Mark L. Witten, R. Clark Lantz, David E. Bice, Bruce A. Muggenburg

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Nedocromil sodium is a nonsteroidal anti-inflammatory drug used to control asthmatic attacks. Our hypothesis is that nedocromil sodium inhibits virus-induced airway inflammation, a common trigger of asthma. We nebulized nedocromil sodium into beagle dogs (n = 10, mean ± SEM ages: 149 ± 13 days) before and after inoculation with canine adenovirus type 2 (CAV2). Control dogs (n = 10) received saline aerosols and were either infected with CAV2 (Sa1/CAV2, n = 7, mean ± SEM ages: 140 ± 11 days) or were not infected (Sal/Sal, n = 3, ages: 143 ± 0 days). All dogs were anesthetized with choralose (80 mg/kg IV), intubated, and mechanically ventilated. Pulmonary function tests and bronchoalveolar lavage (BAL) were performed using standard techniques. Pulmonary function tests revealed no significant change between the nedocromil sodium and non-nedocromil-treated groups. The percentage of infected bronchioles was quantitated as the number of inflamed airways of 40 bronchioles examined times 100 for each dog. Nedocromil-treated dogs had significantly (p < 0.05) less mucosal inflammation (mean ± SEM, 39% ± 5%), epithelial denudation (36% ± 5%), and BAL neutrophilia (11 ± 3) than did Sal/CAV2 dogs (51% ± 6%, 57% ± 4%, and 33% ± 8%, respectively). We concluded that pretreatment with nedocromil sodium aerosols attenuated CAV2-induced airway inflammation in these beagle puppies.

Original languageEnglish (US)
Pages (from-to)317-325
Number of pages9
JournalToxicologic pathology
Volume28
Issue number2
DOIs
StatePublished - Mar 2000

Keywords

  • Nedocromil sodium
  • bronchiolitis
  • inflammation
  • pulmonary function
  • viral infections

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

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    Anderson, K. A., Lemen, R. J., Weger, N. S., Huizhong Chen, N. S., Bowers, M. C., Witten, M. L., Lantz, R. C., Bice, D. E., & Muggenburg, B. A. (2000). Nedocromil Sodium Inhibits Canine Adenovirus Bronchiolitis in Beagle Puppies. Toxicologic pathology, 28(2), 317-325. https://doi.org/10.1177/019262330002800212