Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR

Nafees Ahmad, Sundararajan Venkatesan

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Abstract

In studies of the genetics of human immunodeficiency virus type 1 (HIV-1), the product of the nef gene, formerly known as F, 3′-orf, or B-ORF, was a negative regulator of HIV-1 replication. Proviruses with mutations in the nef gene replicated better than their standard counterparts during transient expression, and the mutant virus maintained its enhanced replication even after serial passages in T lymphocytes. The nef protein trans-suppressed, in a dose-dependent manner, the replication of wild-type and nef mutant proviruses and the expression of reporter genes linked to the HIV-1 long terminal repeat (LTR). The repression induced by the nef protein was mediated by inhibition of transcription from the HIV-1 LTR, which contains a far upstream cis element (previously recognized to be a negative regulatory element) between 340 and 156 nucleotides upstream of the RNA initiation site.

Original languageEnglish (US)
Pages (from-to)1481-1485
Number of pages5
JournalScience
Volume241
Issue number4872
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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