Negative reinforcement reveals non-evoked ongoing pain in mice with tissue or nerve injury

Ying He, Xuebi Tian, Xiaoyu Hu, Frank Porreca, Zaijie Jim Wang

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Patients with chronic pain experience spontaneous or ongoing pain as well as enhanced sensitivity to evoked stimuli. Spontaneous or ongoing pain is rarely evaluated in preclinical studies. In fact, it remains controversial whether ongoing or spontaneous pain even develops in mice after tissue or nerve injury. This study tested a hypothesis that negative reinforcement can be used to unmask the presence of pain in mice with tissue or nerve injury. We found that spinal administration of clonidine or lidocaine did not elicit conditioned place preference (CPP) in uninjured or sham-operated mice. However, these agents produced CPP in mice with chronic inflammation induced by complete Freund's adjuvant (CFA) or following L5/L6 spinal nerve ligation (SNL). These data indicate the presence of non-evoked (ie, stimulus-independent) ongoing pain in mice with chronic inflammation (CFA) or following nerve injury (SNL). In addition, this study validates the use of negative reinforcement to unmask non-evoked ongoing pain in mice. Given the existence of a large collection of transgenic and knockout mice, our data show the application of this approach to elucidate molecular mechanisms underlying non-evoked pain and to contribute to drug discovery for pain. Perspective: We demonstrated the presence of non-evoked ongoing pain in mice with chronic inflammation or following nerve injury. The study also validates the use of negative reinforcement to unmask non-evoked pain in mice. We propose to apply this approach to identify molecular mechanisms and effective drugs for chronic pain.

Original languageEnglish (US)
Pages (from-to)598-607
Number of pages10
JournalJournal of Pain
Volume13
Issue number6
DOIs
StatePublished - Jun 2012

Fingerprint

Nerve Tissue
Pain
Wounds and Injuries
Spinal Nerves
Freund's Adjuvant
Inflammation
Chronic Pain
Ligation
Reinforcement (Psychology)
Clonidine
Drug Discovery
Lidocaine
Knockout Mice
Transgenic Mice

Keywords

  • conditioned place preference
  • inflammatory pain
  • negative reinforcement
  • neuropathic pain
  • Spontaneous pain

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Neurology
  • Clinical Neurology

Cite this

Negative reinforcement reveals non-evoked ongoing pain in mice with tissue or nerve injury. / He, Ying; Tian, Xuebi; Hu, Xiaoyu; Porreca, Frank; Wang, Zaijie Jim.

In: Journal of Pain, Vol. 13, No. 6, 06.2012, p. 598-607.

Research output: Contribution to journalArticle

He, Ying ; Tian, Xuebi ; Hu, Xiaoyu ; Porreca, Frank ; Wang, Zaijie Jim. / Negative reinforcement reveals non-evoked ongoing pain in mice with tissue or nerve injury. In: Journal of Pain. 2012 ; Vol. 13, No. 6. pp. 598-607.
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