Neisseria gonorrhoeae porin P1.B induces endosome exocytosis and a redistribution of Lamp1 to the plasma membrane

Patricia Ayala, Brandi Vasquez, Lee Wetzler, Magdalene "Maggie" So

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The immunoglobulin A (IgA) protease secreted by pathogenic Neisseria spp. cleaves Lamp1, thereby altering lysosomes in a cell and promoting bacterial intracellular survival. We sought to determine how the IgA protease gains access to cellular Lamp1 in order to better understand the role of this cleavage event in bacterial infection. In a previous report, we demonstrated that the pilus-induced Ca2+ transient triggers lysosome exocytosis in human epithelial cells. This, in turn, increases the level of Lamp1 at the plasma membrane, where it can be cleaved by IgA protease. Here, we show that porin also induces a Ca2+ flux in epithelial cells. This transient is similar in nature to that observed in phagocytes exposed to porin. In contrast to the pilus-induced Ca2+ transient, the porin-induced event does not trigger lysosome exocytosis. Instead, it stimulates exocytosis of early and late endosomes and increases Lamp1 on the cell surface. These results indicate that Neisseria pili and porin perturb Lamp1 trafficking in epithelial cells by triggering separate and distinct Ca2+-dependent exocytic events, bringing Lamp1 to the cell surface, where it can be cleaved by IgA protease.

Original languageEnglish (US)
Pages (from-to)5965-5971
Number of pages7
JournalInfection and Immunity
Volume70
Issue number11
DOIs
StatePublished - Nov 2002
Externally publishedYes

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Porins
Neisseria gonorrhoeae
Endosomes
Exocytosis
Immunoglobulin A
Peptide Hydrolases
Lysosomes
Cell Membrane
Neisseria
Epithelial Cells
Phagocytes
Bacterial Infections
Survival

ASJC Scopus subject areas

  • Immunology

Cite this

Neisseria gonorrhoeae porin P1.B induces endosome exocytosis and a redistribution of Lamp1 to the plasma membrane. / Ayala, Patricia; Vasquez, Brandi; Wetzler, Lee; So, Magdalene "Maggie".

In: Infection and Immunity, Vol. 70, No. 11, 11.2002, p. 5965-5971.

Research output: Contribution to journalArticle

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