Neovascularization of the Xenopus embryo

Ondine Cleaver, Kathryn F. Tonissen, Margaret S. Saha, Paul A Krieg

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

The receptor tyrosine kinase, Flk-1 or VEGFR-2, and its ligand, vascular endothelial growth factor (VEGF) are required for the development of the embryonic vasculature. Targeted disruption of either gene in mice results in the failure of vascular system formation. The Xenopus homologues of flk-1 and VEGF have been cloned and their expression has been examined throughout early embryonic development. These studies indicate that flk-1 is expressed in groups of endothelial precursor cells which will form the major blood vessels of the embryo, including the posterior cardinal veins, the dorsal aorta, the vitelline veins, and the endocardium. VEGF expression is found in tissues adjacent to the mesenchyme containing the flk-1- expressing endothelial precursors. Expression of both flk-1 and VEGF is transient, appearing as the primary vascular plexus is forming and declining steadily after the onset of functional embryonic circulation. After establishment of the primary vascular structures, flk-1 expression is also observed in the intersegmental veins which form by an angiogenic mechanism. Overall, these results support a role for VEGF/flk-1 signaling in both vasculogenesis and angiogenesis in the Xenopus embryo. When VEGF is expressed ectopically in Xenopus embryos by microinjection of either plasmid DNA or synthetic mRNA, large, disorganized vascular structures are produced. This result indicates that ectopic VEGF is capable of altering the architecture of the developing vascular network.

Original languageEnglish (US)
Pages (from-to)66-77
Number of pages12
JournalDevelopmental Dynamics
Volume210
Issue number1
DOIs
StatePublished - Sep 1997
Externally publishedYes

Fingerprint

Xenopus
Vascular Endothelial Growth Factor A
Embryonic Structures
Blood Vessels
Veins
Vascular Endothelial Growth Factor Receptor-2
Embryonic Development
Endocardium
Microinjections
Mesoderm
Aorta
Plasmids
Endothelial Cells
Ligands
Messenger RNA
DNA
Genes

Keywords

  • Angiogenesis
  • Flk-1
  • Vascular endothelial cell
  • Vascular endothelial growth factor (VEGF)
  • Vasculogenesis
  • VEGFR- 2

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Neovascularization of the Xenopus embryo. / Cleaver, Ondine; Tonissen, Kathryn F.; Saha, Margaret S.; Krieg, Paul A.

In: Developmental Dynamics, Vol. 210, No. 1, 09.1997, p. 66-77.

Research output: Contribution to journalArticle

Cleaver, Ondine ; Tonissen, Kathryn F. ; Saha, Margaret S. ; Krieg, Paul A. / Neovascularization of the Xenopus embryo. In: Developmental Dynamics. 1997 ; Vol. 210, No. 1. pp. 66-77.
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