Neuroligin 2 regulates spinal GABAergic plasticity in hyperalgesic priming, a model of the transition from acute to chronic pain

Ji Young V Kim, Salim Megat, Jamie K. Moy, Marina N. Asiedu, Galo L. Mejia, Josef Vagner, Theodore J. Price

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Plasticity in inhibitory receptors, neurotransmission, and networks is an important mechanism for nociceptive signal amplification in the spinal dorsal horn. We studied potential changes in GABAergic pharmacology and its underlying mechanisms in hyperalgesic priming, a model of the transition from acute to chronic pain. We find that while GABA A agonists and positive allosteric modulators reduce mechanical hypersensitivity to an acute insult, they fail to do so during the maintenance phase of hyperalgesic priming. In contrast, GABA A antagonism promotes antinociception and a reduction in facial grimacing after the transition to a chronic pain state. During the maintenance phase of hyperalgesic priming, we observed increased neuroligin (nlgn) 2 expression in the spinal dorsal horn. This protein increase was associated with an increase in nlgn2A splice variant mRNA, which promotes inhibitory synaptogenesis. Disruption of nlgn2 function with the peptide inhibitor, neurolide 2, produced mechanical hypersensitivity in naive mice but reversed hyperalgesic priming in mice previously exposed to brain-derived neurotrophic factor. Neurolide 2 treatment also reverses the change in polarity in GABAergic pharmacology observed in the maintenance of hyperalgesic priming. We propose that increased nlgn2 expression is associated with hyperalgesic priming where it promotes dysregulation of inhibitory networks. Our observations reveal new mechanisms involved in the spinal maintenance of a pain plasticity and further suggest that disinhibitory mechanisms are central features of neuroplasticity in the spinal dorsal horn.

Original languageEnglish (US)
Pages (from-to)1314-1324
Number of pages11
JournalPain
Volume157
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Chronic Pain
Maintenance
Hypersensitivity
Pharmacology
GABA-A Receptor Agonists
Neuronal Plasticity
Brain-Derived Neurotrophic Factor
Synaptic Transmission
gamma-Aminobutyric Acid
Pain
Messenger RNA
Peptides
neuroligin 2
Spinal Cord Dorsal Horn
Proteins
Therapeutics

Keywords

  • BDNF
  • Dorsal horn
  • GABA
  • Hyperalgesic priming
  • Neuroligin 2

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology

Cite this

Neuroligin 2 regulates spinal GABAergic plasticity in hyperalgesic priming, a model of the transition from acute to chronic pain. / Kim, Ji Young V; Megat, Salim; Moy, Jamie K.; Asiedu, Marina N.; Mejia, Galo L.; Vagner, Josef; Price, Theodore J.

In: Pain, Vol. 157, No. 6, 01.06.2016, p. 1314-1324.

Research output: Contribution to journalArticle

Kim, Ji Young V ; Megat, Salim ; Moy, Jamie K. ; Asiedu, Marina N. ; Mejia, Galo L. ; Vagner, Josef ; Price, Theodore J. / Neuroligin 2 regulates spinal GABAergic plasticity in hyperalgesic priming, a model of the transition from acute to chronic pain. In: Pain. 2016 ; Vol. 157, No. 6. pp. 1314-1324.
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