Neuropeptide Y Gene Expression Is Increased in the Hypothalamus of Older Women

Carla M. Escobar, Sally J. Krajewski, Tatiana Sandoval-Guzmán, Mary Lou Voytko, Naomi E Rance

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Neuropeptide Y (NPY) and proopiomelanocortin (POMC) neurons in the infundibular (arcuate) nucleus of the hypothalamus are part of a reciprocal circuit regulating reproduction and energy balance. Based on studies showing an age-related decrease in POMC mRNA, we hypothesized that NPY gene expression would increase in older women. In situ hybridization was used to compare NPY mRNA levels between young (premenopausal) and older (postmenopausal) women. We also measured NPY gene expression in intact and ovariectomized young cynomolgus monkeys. We report a significant increase (∼100%) in the numbers of autoradiographic grains/NPY neuron in the retrochiasmatic area and infundibular nucleus of older women. NPY mRNA was correlated with subject age and inversely proportional to the number of POMC neurons previously counted in the same subjects. In contrast, there was no difference in hypothalamic NPY mRNA in intact vs. ovariectomized monkeys. These data show that aging in women is associated with increased NPY gene expression and suggest that the functional relationship between NPY and POMC neurons demonstrated in other species also exists in the human. Our studies of intact and ovariectomized monkeys suggest that the increase in NPY mRNA in older women is due to factors other than the ovarian failure of menopause.

Original languageEnglish (US)
Pages (from-to)2338-2343
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume89
Issue number5
DOIs
StatePublished - May 2004

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Neuropeptide Y
Gene expression
Hypothalamus
Gene Expression
Pro-Opiomelanocortin
Arcuate Nucleus of Hypothalamus
Neurons
Messenger RNA
Haplorhini
Macaca fascicularis
Menopause
Energy balance
In Situ Hybridization
Reproduction
Aging of materials
Networks (circuits)

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Neuropeptide Y Gene Expression Is Increased in the Hypothalamus of Older Women. / Escobar, Carla M.; Krajewski, Sally J.; Sandoval-Guzmán, Tatiana; Voytko, Mary Lou; Rance, Naomi E.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 89, No. 5, 05.2004, p. 2338-2343.

Research output: Contribution to journalArticle

Escobar, Carla M. ; Krajewski, Sally J. ; Sandoval-Guzmán, Tatiana ; Voytko, Mary Lou ; Rance, Naomi E. / Neuropeptide Y Gene Expression Is Increased in the Hypothalamus of Older Women. In: Journal of Clinical Endocrinology and Metabolism. 2004 ; Vol. 89, No. 5. pp. 2338-2343.
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