Neurotensin elevates cytosolic calcium in small cell lung cancer cells

Julie Staley, Gary Fiskum, Thomas P. Davis, Terry W. Moody

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The ability of neurotensin (NT) to elevate cytosolic Ca2+ in small cell lung cancer (SCLC) cells was investigated using the fluorescent Ca2+ indicator Fura 2-AM. Using SCLC cell line NCI-H345, NT elevated cytosolic Ca2+ levels in a concentration-dependent manner. Using a 10 nM dose, NT and C-terminal fragments such as NT(8-13) but not N-terminal fragments such as NT(1-8) elevated the cytosolic Ca2+ levels. Because EGTA (5 mM) did not affect the NT response, NT may cause release of Ca2+ from intracellular stores. These data indicate that SCLC NT receptors may use Ca2+ as a second messenger.

Original languageEnglish (US)
Pages (from-to)1217-1221
Number of pages5
JournalPeptides
Volume10
Issue number6
DOIs
StatePublished - 1989

Keywords

  • Cytosolic calcium
  • Neurotensin
  • Small cell lung cancer

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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