Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum

E. W. Uhl, R. M. O'Connor, L. E. Perryman, Michael W Riggs

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11 Citations (Scopus)

Abstract

Isolates of Cryptosporidium parvum from New York, Florida, Brazil, Mexico, and Peru were examined for the presence of two sporozoite surface epitopes originally identified in an Iowa isolate by neutralizing monoclonal antibodies (MAbs) 18.44 and 17.41. Immunofluorescence microscopy and immunoblotting demonstrated the presence of both epitopes on all isolates. Incubation of DEAE-cellulose-purified sporozoites of the New York, Florida, Brazil, and Mexico isolates with MAb 18.44 or 17.41 significantly neutralized their infectivity for 4- to 6-day-old BALB/c mice. The results indicate that two neutralization-sensitive epitopes are conserved on geographically diverse C. parvum isolates.

Original languageEnglish (US)
Pages (from-to)1703-1706
Number of pages4
JournalInfection and Immunity
Volume60
Issue number4
StatePublished - 1992

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Cryptosporidium parvum
Epitopes
Sporozoites
Mexico
Brazil
DEAE-Cellulose
Peru
Neutralizing Antibodies
Fluorescence Microscopy
Immunoblotting
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum. / Uhl, E. W.; O'Connor, R. M.; Perryman, L. E.; Riggs, Michael W.

In: Infection and Immunity, Vol. 60, No. 4, 1992, p. 1703-1706.

Research output: Contribution to journalArticle

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