Neutrophil activation subsequent to cardiopulmonary bypass: A sensitive method for determining neutrophil activation by quantification of lactoferrin concentrations

P. J. McQueen, M. C. Bowers, Douglas F Larson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Neutrophils have been implicated as contributors to the pathogenesis of cardiopulmonary bypass (CPB). We report a method to quantify the extent of neutrophil activation during CPB through measuring the serum concentrations of lactoferrin. We developed a modified enzyme-linked immunosorbent assay (ELISA) for the quantification of lactoferrin that is sensitive to 1 ng/ml. The preliminary data acquired to support this method confirm that there is a time-dependent increase in neutrophil activity during CPB. This was determined by measuring lactoferrin serum concentrations, and the lactoferrin concentrations appear to be higher in the pediatric patient compared to the adult patient.

Original languageEnglish (US)
Pages (from-to)11-14
Number of pages4
JournalJournal of Extra-Corporeal Technology
Volume27
Issue number1
StatePublished - 1995

Fingerprint

Neutrophil Activation
Lactoferrin
Cardiopulmonary Bypass
Neutrophils
Serum
Enzyme-Linked Immunosorbent Assay
Pediatrics

Keywords

  • cardiopulmonary bypass
  • ELISA
  • lactoferrin
  • neutrophil

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

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T1 - Neutrophil activation subsequent to cardiopulmonary bypass

T2 - A sensitive method for determining neutrophil activation by quantification of lactoferrin concentrations

AU - McQueen, P. J.

AU - Bowers, M. C.

AU - Larson, Douglas F

PY - 1995

Y1 - 1995

N2 - Neutrophils have been implicated as contributors to the pathogenesis of cardiopulmonary bypass (CPB). We report a method to quantify the extent of neutrophil activation during CPB through measuring the serum concentrations of lactoferrin. We developed a modified enzyme-linked immunosorbent assay (ELISA) for the quantification of lactoferrin that is sensitive to 1 ng/ml. The preliminary data acquired to support this method confirm that there is a time-dependent increase in neutrophil activity during CPB. This was determined by measuring lactoferrin serum concentrations, and the lactoferrin concentrations appear to be higher in the pediatric patient compared to the adult patient.

AB - Neutrophils have been implicated as contributors to the pathogenesis of cardiopulmonary bypass (CPB). We report a method to quantify the extent of neutrophil activation during CPB through measuring the serum concentrations of lactoferrin. We developed a modified enzyme-linked immunosorbent assay (ELISA) for the quantification of lactoferrin that is sensitive to 1 ng/ml. The preliminary data acquired to support this method confirm that there is a time-dependent increase in neutrophil activity during CPB. This was determined by measuring lactoferrin serum concentrations, and the lactoferrin concentrations appear to be higher in the pediatric patient compared to the adult patient.

KW - cardiopulmonary bypass

KW - ELISA

KW - lactoferrin

KW - neutrophil

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