New approaches for bioaugmentation as a remediation technology

Terry J. Gentry, Christopher Rensing, Ian L. Pepper

Research output: Contribution to journalReview article

185 Scopus citations

Abstract

Bioaugmentation is commonly employed as a remediation technology. However, numerous studies indicate that introduced microorganisms often do not survive in the environment and thus do not increase contaminant remediation. This review details several new approaches that may increase the persistence and activity of exogenous microorganisms and/or genes following introduction into the environment. These techniques include: (1) bioaugmentation with cells encapsulated in a carrier such as alginate; (2) gene bioaugmentation where the goal is for the added inoculant to transfer remediation genes to indigenous microorganisms; (3) rhizosphere bioaugmentation where the microbial inoculant is added to the site along with a plant that serves as a niche for the inoculant's growth; and (4) phytoaugmentation where the remediation genes are engineered directly into a plant for use in remediation without a microbial inoculant. Additionally, the review discusses the generation of genetically engineered microorganisms for use in bioaugmentation along with methods for the control of the engineered microorganisms in the environment, and the potential effects of the release on indigenous organisms. Various methods for the detection of introduced microorganisms such as real-time polymerase chain reaction (PCR) and reporter genes are also addressed. Ultimately, these new approaches may broaden the application of bioaugmentation as a remediation technology.

Original languageEnglish (US)
Pages (from-to)447-494
Number of pages48
JournalCritical Reviews in Environmental Science and Technology
Volume34
Issue number5
DOIs
StatePublished - Sep 15 2004

Keywords

  • Genetically engineered microorganism
  • Immobilized cell
  • Phytoaugmentation
  • Phytoremediation
  • Reporter gene
  • Suicide gene

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

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