New cloning vectors using benomyl resistance as a dominant marker for selection inNeurospora crassa and in other filamentous fungi

C. Robertson McClung, John D. Phillips, Marc J. Orbach, Jay C. Dunlap

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We describe five new plasmid vectors derived from pBR322 that carry theNeurospora crassa β-tubulin gene conferring resistance to benomyl. The benomyl resistance gene has been modified to eliminate an internalEcoRI site to facilitate the cloning ofEcoRI restriction fragments. These plasmids allow rapid subcloning of fragments from one replicon to another without insert fragment purification due to the presence of different drug resistance markers (resistance to kanamycin, tetracycline, or chloramphenicol) carried on the plasmids. These vectors will allow rapid transformation ofN. crassa and other filamentous fungi to allow phenotypic characterization of subcloned fragments.

Original languageEnglish (US)
Pages (from-to)299-302
Number of pages4
JournalExperimental Mycology
Volume13
Issue number3
DOIs
StatePublished - Sep 1989
Externally publishedYes

Keywords

  • Neurospora
  • benomyl
  • filamentous fungi
  • pBR322
  • plasmids
  • subcloning
  • transformation
  • vectors
  • β-tubulin

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'New cloning vectors using benomyl resistance as a dominant marker for selection inNeurospora crassa and in other filamentous fungi'. Together they form a unique fingerprint.

Cite this