New molecular approaches to tissue analysis

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The completion of the Human Genome Project will produce new opportunities for analysis of genes and their products in human tissue. The emergence of new technologies will enable investigators to directly examine human tissues for gene deletion, transposition, and amplification. In addition, we will be able to assess the complete gene expression of a tissue by examining the mRNA species using microarray chips. The emerging technologies of laser capture microdissection and RNA amplification enables these procedures to be carried out on groups of a few hundred cells, which will facilitate the examination of heterogeneous lesions. Finally, the application of tissue arrays and the capability of obtaining protein sequences in samples of only a few femtomoles of protein using desorption mass spectroscopy will revolutionize the analysis of protein expression.

Original languageEnglish (US)
Pages (from-to)1063-1064
Number of pages2
JournalJournal of Histochemistry and Cytochemistry
Volume49
Issue number8
StatePublished - 2001

Fingerprint

Laser Capture Microdissection
Human Genome Project
Technology
Proteins
Gene Amplification
Gene Deletion
Mass Spectrometry
Research Personnel
RNA
Gene Expression
Messenger RNA
Genes

Keywords

  • Genes
  • Human Genome Project
  • mRNA
  • Protein sequences

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

New molecular approaches to tissue analysis. / Nagle, Raymond B.

In: Journal of Histochemistry and Cytochemistry, Vol. 49, No. 8, 2001, p. 1063-1064.

Research output: Contribution to journalArticle

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