Next-generation transgenic cotton: Pyramiding RNAi and Bt counters insect resistance

Mi Ni, Wei Ma, Xiaofang Wang, Meijing Gao, Yan Dai, Xiaoli Wei, Lei Zhang, Yonggang Peng, Shuyuan Chen, Lingyun Ding, Yue Tian, Jie Li, Haiping Wang, Xiaolin Wang, Guowang Xu, Wangzhen Guo, Yihua Yang, Yidong Wu, Shannon Heuberger, Bruce E TabashnikTianzhen Zhang, Zhen Zhu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.

Original languageEnglish (US)
JournalPlant Biotechnology Journal
DOIs
StateAccepted/In press - 2017

Fingerprint

Bacillus thuringiensis
RNA Interference
RNA interference
Insects
cotton
genetically modified organisms
insects
Juvenile Hormones
juvenile hormones
toxins
Helicoverpa armigera
Genetically Modified Plants
methyltransferases
Methyltransferases
binding proteins
Carrier Proteins
pests
Gossypium
insecticidal proteins
pest resistance

Keywords

  • Bacillus thuringiensis
  • Helicoverpa armigera
  • Genetic engineering
  • Juvenile hormone
  • RNA interference
  • Sustainability

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Next-generation transgenic cotton : Pyramiding RNAi and Bt counters insect resistance. / Ni, Mi; Ma, Wei; Wang, Xiaofang; Gao, Meijing; Dai, Yan; Wei, Xiaoli; Zhang, Lei; Peng, Yonggang; Chen, Shuyuan; Ding, Lingyun; Tian, Yue; Li, Jie; Wang, Haiping; Wang, Xiaolin; Xu, Guowang; Guo, Wangzhen; Yang, Yihua; Wu, Yidong; Heuberger, Shannon; Tabashnik, Bruce E; Zhang, Tianzhen; Zhu, Zhen.

In: Plant Biotechnology Journal, 2017.

Research output: Contribution to journalArticle

Ni, M, Ma, W, Wang, X, Gao, M, Dai, Y, Wei, X, Zhang, L, Peng, Y, Chen, S, Ding, L, Tian, Y, Li, J, Wang, H, Wang, X, Xu, G, Guo, W, Yang, Y, Wu, Y, Heuberger, S, Tabashnik, BE, Zhang, T & Zhu, Z 2017, 'Next-generation transgenic cotton: Pyramiding RNAi and Bt counters insect resistance', Plant Biotechnology Journal. https://doi.org/10.1111/pbi.12709
Ni, Mi ; Ma, Wei ; Wang, Xiaofang ; Gao, Meijing ; Dai, Yan ; Wei, Xiaoli ; Zhang, Lei ; Peng, Yonggang ; Chen, Shuyuan ; Ding, Lingyun ; Tian, Yue ; Li, Jie ; Wang, Haiping ; Wang, Xiaolin ; Xu, Guowang ; Guo, Wangzhen ; Yang, Yihua ; Wu, Yidong ; Heuberger, Shannon ; Tabashnik, Bruce E ; Zhang, Tianzhen ; Zhu, Zhen. / Next-generation transgenic cotton : Pyramiding RNAi and Bt counters insect resistance. In: Plant Biotechnology Journal. 2017.
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abstract = "Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.",
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AU - Ni, Mi

AU - Ma, Wei

AU - Wang, Xiaofang

AU - Gao, Meijing

AU - Dai, Yan

AU - Wei, Xiaoli

AU - Zhang, Lei

AU - Peng, Yonggang

AU - Chen, Shuyuan

AU - Ding, Lingyun

AU - Tian, Yue

AU - Li, Jie

AU - Wang, Haiping

AU - Wang, Xiaolin

AU - Xu, Guowang

AU - Guo, Wangzhen

AU - Yang, Yihua

AU - Wu, Yidong

AU - Heuberger, Shannon

AU - Tabashnik, Bruce E

AU - Zhang, Tianzhen

AU - Zhu, Zhen

PY - 2017

Y1 - 2017

N2 - Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.

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KW - Genetic engineering

KW - Juvenile hormone

KW - RNA interference

KW - Sustainability

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