NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

Clement Lin, Jonathan Dickerhoff, Danzhou Yang

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

G-quadruplexes are noncanonical, four-stranded nucleic acid secondary structures formed in sequences containing consecutive runs of guanines. These G-quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for understanding the structures of G-quadruplexes and their interactions with small molecules under physiologically relevant conditions. Here, we present the NMR methodology used in our research group for the study of DNA G-quadruplex structures in physiologically relevant solution and their ligand interactions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages157-176
Number of pages20
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2035
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • G-quadruplex structures
  • G-quadruplex-interactive compounds
  • NMR spectroscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Lin, C., Dickerhoff, J., & Yang, D. (2019). NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds. In Methods in Molecular Biology (pp. 157-176). (Methods in Molecular Biology; Vol. 2035). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9666-7_9