NMR studies of protein-RNA interactions

Carla A. Theimer, Nakesha L. Smith, May Khanna

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

This chapter describes the preparation of NMR quantities of RNA purified to single-nucleotide resolution for protein-RNA interaction studies. The protocol is easily modified to make nucleotide-specific isotopically labeled RNAs or uniformly labeled RNA fragments for ligation to generate segmentally labeled RNAs.

Original languageEnglish (US)
Title of host publicationProtein NMR Techniques
Pages197-218
Number of pages22
DOIs
StatePublished - Jan 20 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume831
ISSN (Print)1064-3745

Keywords

  • In vitro transcription
  • Isotopic labeling
  • Protein-RNA interactions
  • RNA purification
  • RNA synthesis
  • Single-nucleotide resolution

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Theimer, C. A., Smith, N. L., & Khanna, M. (2012). NMR studies of protein-RNA interactions. In Protein NMR Techniques (pp. 197-218). (Methods in Molecular Biology; Vol. 831). https://doi.org/10.1007/978-1-61779-480-3_12